Abstract
We investigated the adaptative response of S. cerevisiae in sod mutants (sod1Δ, sod2Δ and sod1Δsod2Δ) after H2O2 treatment in the stationary phase. sod2Δ and sod1Δsod2Δ demonstrated the highest levels of GSH in the control, suggesting that pathways which include GSH protect these double mutants against oxidative stress. In addition, sod1Δ and sod1Δsod2Δ had higher iron levels than the wild-type, independently of H2O2 stress. Fe levels were increased in sod2Δ following H2O2 In addition, the sod2Δ mutant was more sensitive to H2O2 treatment than the wild-type. These results suggest that sod2Δ sensibility may be associated with •OH production by the Fenton reaction. This increased iron demand in the sod2Δ mutant may be a reflection of the cells’ efforts to reconstitute proteins that are inactivated in conditions of excess superoxide. MDA levels were assayed by HPLC in these mutants. The highest MDA levels could be observed after 10mM H2O2 treatment in the sod1Δsod2Δ double mutant. After treatment with a GSH inhibitor, the MDA level was still higher in the same strain. Thus, both direct and indirect GSH pathways are involved in the protection of lipid membranes and proteins in these mutants and may constitute an adaptative response to enhanced basal oxidative damage produced by superoxide.
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Manfredini, V., Martins, V.D., Peralba, M.d.C.R. et al. Adaptative response to enhanced basal oxidative damage in sod mutants from Saccharomyces cerevisiae. Mol Cell Biochem 276, 175–181 (2005). https://doi.org/10.1007/s11010-005-4058-0
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DOI: https://doi.org/10.1007/s11010-005-4058-0