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Time course studies on the initiation of complement activation in acute myocardial infarction induced by coronary artery ligation in rats

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Abstract

This study attempted to probe the role of complement activation in promoting acute myocardial infarction (AMI) induced by coronary artery ligation (CAL) in rats. The surgical technique used in this study significantly reduced early mortality (95% survival rate) and also reduced the variation in infarct size (33± 1.87%) at 32 h after surgery. Time course studies on the initiation of AMI at various time points were carried out using physiological, biochemical, histopathological and electron microscopical techniques. Serum markers and activities of lysosomal hydrolases were found to be significantly elevated at the 8th hour post ligation. Histological studies showed polymorphonuclear cells emigration and total coagulation necrosis. Transmission electron micrograph exhibited mild distortion of muscle fibres and mitochondrial rupture with disrupted cristae. Immunoblotting studies confirmed the presence of α2-macroglobulin which supported the inflammatory response at 8th h of post ligation. The initiation of the complement (C) activation was observed by the increase in the level of the soluble form of the membrane attack complex (sC5b-9) in serum and left ventricle. Immunoexpression studies confirmed the initiation of the terminal C activation as shown by the expression of C5, C6, C7, C8, C9 and sC5b-9 complex at the 8th h of AMI. This study conclusively demonstrated that initiation of the C activation was observed to be significant at the 8th h of AMI induced by CAL in rats. (Mol Cell Biochem 268: 149–158, 2005)

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Authors and Affiliations

  1. Department of Biotechnology, Central Leather Research Institute, Adyar, Chennai, India

    Miriyala Sumitra, Panchatcharam Manikandan & Rengarajulu Puvanakrishnan Dr.

  2. Alved Pharma & Foods Pvt. Ltd., Chennai, India

    Mohammed Nayeem

  3. Department of Pathology, Madras Veterinary College, Vepery, Chennai, India

    Bhakthavatsalam Murali Manohar & Subbiah Vairamuthu

  4. Department of Biophysics and Electron Microscopy, Central Leather Research Institute, Adyar, Chennai, India

    Beema Lokanadam

  5. Department of Biochemistry, Apollo Hospitals, Greams Lane, Chennai, India

    Samu Subramaniam

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  1. Miriyala Sumitra
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  2. Panchatcharam Manikandan
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  3. Mohammed Nayeem
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  4. Bhakthavatsalam Murali Manohar
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  8. Rengarajulu Puvanakrishnan Dr.
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Correspondence to Rengarajulu Puvanakrishnan Dr..

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Sumitra, M., Manikandan, P., Nayeem, M. et al. Time course studies on the initiation of complement activation in acute myocardial infarction induced by coronary artery ligation in rats. Mol Cell Biochem 268, 149–158 (2005). https://doi.org/10.1007/s11010-005-3856-8

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  • DOI: https://doi.org/10.1007/s11010-005-3856-8

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