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Thyroid hormones stimulate Na+–Pi transport activity in rat renal brush-border membranes: Role of membrane lipid composition and fluidity

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Abstract

Antecedent studies have suggested that lipid composition and fluidity of cellular membranes of various organs are altered in response to thyroid hormone status. To date, the effects of thyroid hormone status on these parameters have not been examined in rat renal apical membrane in regard to sodium-dependent phosphate transport. In the present study, we determined the potential role of alterations in cortical brush-border membrane lipid composition and fluidity in modulation of Na+–Pi transport activity in response to thyroid hormone status. Thyroid hormone status influences the fractional excretion of Pi, which is associated with alteration in renal brush-border membrane phosphate transport. The increment in Na+–Pi transport in renal BBMV isolated from Hyper-T rats is manifested as an increase in the maximal velocity (Vmax) of Na+–Pi transport. Further, the cholesterol content was significantly increased in renal BBM of Hypo-T rats and decreased in Hyper-T rats as compared to the Eu-T rats. The molar ratio of cholesterol/phospholipids was also higher in renal BBM from hypo-T rats. Subsequently, fluorescence anisotropy of diphenyl hexatriene (rDPH) and microviscosity were significantly decreased in the renal BBM of the Hyper-T rats and increased in the Hypo-T rats as compared to Eu-T rats. The result of this study, therefore, suggest that alteration in renal BBM cholesterol, cholesterol/phospholipid molar ratio, and membrane fluidity play an important role in the modulation of renal BBM Na+–Pi transport in response to thyroid hormone status of animals. (Mol Cell Biochem 268: 75–82, 2005)

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References

  1. Jacobowitz IE, Pang KY, Harmats PR, Walker WA: Structural and functional maturation of rat gastrointestinal barrier with thyroxine. Am J Physiol 252: G762–G767, 1987

    Google Scholar 

  2. Kobori H, Ichihara A, Miyashita Y, Saula T: Mechanism of hyper thyroidism induced renal hypertrophy in rats. J Endocrinol 159: 9–14, 1998

    Article  Google Scholar 

  3. Mundy GR, Raisz LG: Thyrotoxicosis and calcium metabolism. Miner Electrolyte Metab 2: 285–292, 1979

    Google Scholar 

  4. Monternegro J, Gonzales O, Saracho R, Aguirre R, Martinez I: Changes in renal function in primary hypothyroidism. Am J Kid Dis 27: 195–198, 1986

    Google Scholar 

  5. Yusufi ANK, Murayama N, Killer MJ, Dousa TP: Modulatory effect of thyroid hormones on uptake of phosphate and other solutes across luminal brush border membrane of kidney cortex. Endocrinology 116: 3606–3610, 1985

    Google Scholar 

  6. Kinsella JL, Sacktor B: Thyroid hormones increase Na+–H+ exchange activity in renal brush border membrane proc. Proc Natl Acad Sci (USA) 8: 3606–3610, 1985

    Google Scholar 

  7. Hoch FL: Lipids and thyroid hormones. Prog Lipid Res 27: 199–270, 1988

    Article  Google Scholar 

  8. Benga G, Homes RP: Interaction between components in biological membranes and their implications for membrane function. Prog Biophys Mol Biol 43: 195–257, 1984

    Article  Google Scholar 

  9. Brasitus TA, Schaechter D, Mamouneas TG: Functional interactions of lipids and proteins in rat intestinal microvillus membranes. Biochemistry 18: 4136–4144, 1979.

    Google Scholar 

  10. Proulx P: Structure–function relationship in intestinal brush border membranes. Biochim Biophys Acta 1071: 255–271, 1991

    Google Scholar 

  11. Levi M, Jameson DM, Wieb B, Van Der Meer B: Role of BBM lipid composition and fluidity in impaired renal Pi transport in aged rats. Am J Physiol 256: F85–F94, 1989

    Google Scholar 

  12. Brasitus TA, Dudeja PK: Effect of Hypothyroidism on the lipid composition and fluidity of rat colonic apical plasma membrane. Biochim Biophys Acta 939: 189–196, 1988

    Google Scholar 

  13. Abrums JJ, Grundy SM: Cholesterol metabolism in hypo and hyperthyroidism in man. Lipid J Res 822: 323–338, 1981

    Google Scholar 

  14. Kussi TMR, Taskinon MR, Nikkla EA: Lipoproteins, lipolytic enzymes and hormonal status in hypothroid women at different level of substitution. Clin J Endocrinol Metabol 66: 57–66, 1998

    Google Scholar 

  15. Prasad R, Kumar V, Kumar R, Singh KP: Thyroid hormones modulate zinc transport activity of rat intestinal and renal brush border membrane. Am J Physiol 276: E774–E782, 1999

    Google Scholar 

  16. Prasad R, Kinsella JL, Sacktor B: Renal adaptation to metabolic acidosis in the senescent rat. Am J Physiol 255: F1183–F119, 1988

    Google Scholar 

  17. Prasad R, Kaur D, Kumar V: Kinetic characterization of zinc binding to brush border membranes from rat kidney cortex. Interaction with cadmium. Biochim Biophys Acta 1284: 69–78, 1996

    Google Scholar 

  18. Peterson GL: A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem 83: 346–356 1977

    CAS  PubMed  Google Scholar 

  19. Ames BN, Dubin DT: The role of polyamines in the neutralization of bacteriophage of deoxyribonucleic acid. J Biol Chem 235: 769–775, 1960

    Google Scholar 

  20. Esko JD, Raetz CRH: Mutants of Chinese hamster ovary cells with altered membrane phospholipid composition. J Biol Chem 255: 4474–4480, 1980

    Google Scholar 

  21. Zlatkis A, Zak B, Boyle A: A new method for the direct determination of serum cholesterol. J Lab Clin Med 42: 486–492, 1953

    Google Scholar 

  22. Shinitzky M, Barenholz Y: Fluidity parameters of lipid regions determined by fluorescence polarization. Biochim Biophys Acta 515: 367–394, 1978

    Google Scholar 

  23. Guyton AC, Hall JE: The thyroid metabolic hormones. In: A.L. Guyton and J.E. Halls (eds). Text Book of Medical Physiology, Saunders, Philadelphia, PA, 1996, pp 945–956

    Google Scholar 

  24. Hodin RA, Lazar MA, Wintman B: Identification of thyroid hormone receptor that is pitutary specific. Science 244: 76–79, 1989

    Google Scholar 

  25. Hodin RA, Shei A, Morin M, Meng S: Thyroid hormone and the gut: Selective transcriptional activation of villus enterocyte marker. Surgery 120: 138–143, 1996

    Google Scholar 

  26. Weinberger CC, Thomson C, Orag ES, Lebo R, Grecol DJ, Evans RM: The C-erb A gene encodes a thyroid hormone receptor. Nature 324: 641–646, 1986

    Google Scholar 

  27. Espinosa RE, Killer MJ, Yusufi ANK, Dousa T: Effect of thyroxin on phosphate transport across renal cortical brush border membrane. Am J Physiol 246: F133–F139, 1984

    Google Scholar 

  28. Beers KW, Dousa TP: Thyroid hormone stimulates the Na+–PO4 symporter but not Na+–SO4 symporter in renal brush border. Am J Physiol 265: F323–F326, 1993

    Google Scholar 

  29. Van Bliterswijk WJ, Vander Meer BW, Hilkmann H: Quantitative contributions of cholesterol and the individual classes of phospholipids and their degree of fatty acyl unsaturation to membrane fluidity measured by fluorescence polarization. Biochemistry 26: 1746–1756, 1987

    Google Scholar 

  30. Friedlander G, Shamedi M, Le Grimellec C, Amiel C: Increase in membrane fluidity and opening of tight junction have similar effects on sodium-coupled uptakes in renal epithelial cells. J Biol Chem 26: 11183–11188, 1988

    Google Scholar 

  31. Molitoris BA, Alfrey AC, Harris RA, Simon FR: Renal apical membrane cholesterol and fluidity is regulation of phosphate transport. Am J Physiol 249: F12–F19, 1985

    Google Scholar 

  32. Yusufi ANK, Murayama NB, Killer MJ, Dousa TD: Different mechanisms of adaptive increase in Na+–Pi co-transport across renal brush border membrane. Am J Physiol 256: F852–F861, 1989

    Google Scholar 

  33. Spector AA, Yorek MA: Membrane lipid composition and cellular function. J Lipid Res 26: 1015–1035, 1985

    Google Scholar 

  34. Smedt De H, Kinne R: Temperature dependence of solute transport and enzyme activities in hog renal brush border membrane vesicles. Biochim Biophys Acta 648: 247–253, 1981

    Google Scholar 

  35. Hooper NM, Turner AJ: Ectoenzymes of the kidney microviliar membrane. Differential solubilization by detergents can predict a glycosyl-phosphatidyl inositol membrane anchor. J Biochem 250: 865–869, 1988.

    Google Scholar 

  36. Lowe MG, Saltiel AR: Structural and functional roles of glycosyl-phosphatidyl inositol in membranes. Science 239: 268–275, 1988

    CAS  PubMed  Google Scholar 

  37. Kumar V, Prasad R: Molecular basis of renal handling of calcium in response to thyroid hormone status of rat. Biochim Biophys Acta 1586: 331–343, 2002

    Google Scholar 

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  1. Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India

    Rajendra Prasad Dr. & Vivek Kumar

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  1. Rajendra Prasad Dr.
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  2. Vivek Kumar
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Correspondence to Rajendra Prasad Dr..

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Prasad, R., Kumar, V. Thyroid hormones stimulate Na+–Pi transport activity in rat renal brush-border membranes: Role of membrane lipid composition and fluidity. Mol Cell Biochem 268, 75–82 (2005). https://doi.org/10.1007/s11010-005-3545-7

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