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Amino Acid Composition, Antioxidant, α-Glucosidase and α-Amylase Inhibitory Activities of the Enzymic Polypeptide from Acer truncatum Seed Meal

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Abstract

As a new food raw material, Acer truncatum seed has been used in the production of edible oil. However, the research on the processing residue (seed meal) has not been reported. The Acer truncatum seed meal was hydrolyzed by seven enzymes and the enzymic polypeptides obtained were characterized by thermogravimetric analysis, gel permeation chromatography, fourier transform infrared spectroscopy and zeta pals dynamic light scattering. In addition, the degree of hydrolysis, amino acid composition, antioxidant activity, α-glucosidase inhibition activity and α-amylase inhibition activity of enzymatic polypeptide were evaluated. The results showed that the yields, average molecular weights, average particle sizes and zeta potentials of enzymic polypeptides obtained were 20.5–40.2%, 7.8–13.9 kDa, 288.3–997.5 nm and ( −)1.8 to ( −)36.4 mV, respectively. The bioactivity results showed that the enzymatic polypeptides not only had higher antioxidant activity, but also better α-glucosidase and α-amylase inhibition activities than the raw materials. Among the seven enzymatic polypeptides, the alkaline protease enzymatic polypeptide had the highest content of the eight essential amino acids (20.3%), the largest value of hydrolysis degree (15.4%) and the most significant bioactivity. The study will provide the data support for developing the new application of seed meal from Acer truncatum.

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Data Availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

References

  • Aralbaeva AN, Mamataeva AT, Zhaparkulova NI, Utegalieva RS, Khanin M, Danilenko M, Murzakhmetova MK (2017) A composition of medicinal plants with an enhanced ability to suppress microsomal lipid peroxidation and a protective activity against carbon tetrachloride-induced hepatotoxicity. Biomed Pharmacother 96:1283–1291

    Article  CAS  Google Scholar 

  • Artimo P, Jonnalagedda M, Arnold K, Baratin D, Csardi G, Castro E, Duvaud S, Flegel V, Fortier A, Gasteiger E, Grosdidier A, Hernandez C, Ioannidis V, Kuznetsov D, Liechti R, Moretti S, Mostaguir K, Redaschi N, Rossier G, Stockinger H (2012) ExPASy: SIB bioinformatics resource portal. Nucleic Acids Res 40:W597-603

    Article  CAS  Google Scholar 

  • Baynes JW (1991) Role of oxidative stress in development of complications in diabetes. Diabetes 40:405–412

    Article  CAS  Google Scholar 

  • Bharatham K, Bharatham N, Park K, Lee K (2008) Binding mode analyses and pharmacophore model development for sulfonamide chalcone derivatives, a new class of α-glucosidase inhibitors. J Mol Graph Model 26:1202–1212

    Article  CAS  Google Scholar 

  • Bi W, Gao Y, Shen J, He CN, Liu HB, Peng Y, Zhang CH, Xiao PG (2016) Traditional uses, phytochemistry, and pharmacology of the genus Acer (maple): a review. J Ethnopharmacol 189:31–60

    Article  CAS  Google Scholar 

  • Calin M, Constantinescu-Aruxandei D, Alexandrescu E, Raut I, Doni MB, Arsene ML, Oancea F, Jecu L, Lazar V (2017) Degradation of keratin substrates by keratinolytic fungi. Electron J Biotechnol 28:101–112

    Article  CAS  Google Scholar 

  • Ding XL, Wei BQ, Deng MG, Chen H, Shan ZH (2022) Effect of protein peptides with different molecular weights on the setting and hydration process of gypsum. Constr Build Mater 318:126185

    Article  CAS  Google Scholar 

  • Erukainure OL, Onifade OF, Odjobo BO, Olasehinde TA, Adesioye TA, Tugbobo-Amisu AO, Adenekan SO, Okonrokwo GI (2018) Ethanol extract of Tetrapleura tetraptera fruit peels: chemical characterization, and antioxidant potentials against free radicals and lipid peroxidation in hepatic tissues. J Taibah Univ Sci 11:861–867

    Article  Google Scholar 

  • Frenkel O, Bivas-Benita M, Nassar T, Springer C, Sherman Y, Avital A, Altschuler Y, Borlak J, Benita S (2010) A safety and tolerability study of differently-charged nanoparticles for local pulmonary drug delivery. Toxicol Appl Pharm 246:83–90

    Article  Google Scholar 

  • Gu RH, Morcol T, Liu B, Shi MJ, Kennelly EJ, Long CL (2019) GC–MS, UPLC-QTOF-MS, and bioactivity characterization of Acer truncatum seeds. Ind Crop Prod 138:111480

    Article  CAS  Google Scholar 

  • Hsu B, Weng Y, Liao Y, Chen W (2005) Structural investigation of edible zein films/coatings and directly determining their thickness by FT-Raman spectroscopy. J Agric Food Chem 53:5089–5095

    Article  CAS  Google Scholar 

  • Hu P, Li D, Jia C, Liu Q, Wang X, Li Z, Hua H (2017) Bioactive constituents from Vitex negundo var. heterophylla and their antioxidant and α-glucosidase inhibitory activities. J Funct Foods 35:236–244

    Article  CAS  Google Scholar 

  • Jin W, Wu H, Zhu B, Ran X (2012) Functional properties of gelation-like protein hydrolysates from scallop (Patinopecten yessoensis) male gonad. Eur Food Res Technol 234:863–872

    Article  CAS  Google Scholar 

  • Kavanagh G, Clark A, Ross-Murphy S (2000) Heat-induced gelation of globular proteins: Part 3. Molecular studies on low pH b-lactoglobulin gels. Int J Bio Macromol 28:41–50

    Article  CAS  Google Scholar 

  • Kong J, Yu S (2007) Fourier transform infrared spectroscopic analysis of protein secondary structures. Acta Biochem Biophys Sin 39:549–559

    Article  CAS  Google Scholar 

  • Li Q, Chen J, Yu XZ, Gao JM (2019) A mini review of nervonic acid: source, production, and biological functions. Food Chem 301:125286

    Article  CAS  Google Scholar 

  • Liang XX, Gao YY, Fei WB, Zou YF, He M, Yin ZL, Yuan ZX, Yin ZQ, Zhang W (2018) Chemical characterization and antioxidant activities of polysaccharides isolated from the stems of Parthenocissus tricuspidate. Int J Biol Macromol 119:70–78

    Article  CAS  Google Scholar 

  • Liu J, Jin Y, Lin S, Jones GS, Chen F (2015) Purification and identification of novel antioxidant peptides from egg white protein and their antioxidant activities. Food Chem 175:258–266

    Article  CAS  Google Scholar 

  • López Barón N, Gu Y, Vasanthan T, Hoover R (2017a) Plant proteins mitigate in vitro wheat starch digestibility. Food Hydrocoll 69:19–27

    Article  Google Scholar 

  • López Barón N, Sagnelli D, Blennow A, Holse M, Gao J, Saaby L, Müllertz A, Møller Jespersen B, Vasanthan T (2017b) Hydrolysed pea proteins mitigate in vitro wheat starch digestibility. Food Hydrocoll 79:117–126

    Article  Google Scholar 

  • Lu SH, Zuo HJ, Shi JX, Li CR, Li YH, Wang X, Li LR, Huang J (2019) Two new glycosides from the leaves of Ligustrum robustum and their antioxidant activities and inhibitory effects on α-glucosidase and α-amylase. S Afr J Bot 125:521–526

    Article  CAS  Google Scholar 

  • Marcilla A, Gomez-Siurana A, Gomis C, Chapuli E, Carmen CM, Valdes FJ (2009) Characterization of microalgal species through TGA/FTIR analysis: application to nannochloropsis sp. Thermochim Acta 484:41–47

    Article  CAS  Google Scholar 

  • Merkel O, Zheng M, Debus H, Kissel T (2012) Pulmonary gene delivery using polymeric nonviral vectors. Bioconjugate Chem 23:3–20

    Article  CAS  Google Scholar 

  • Musa KH, Abdullah A, Al-Haiqi A (2016) Determination of DPPH free radical scavenging activity: application of artificial neural networks. Food Chem 194:705–711

    Article  CAS  Google Scholar 

  • Ngoh Y, Gan C (2016) Enzyme-assisted extraction and identification of antioxidative and α-amylase inhibitory peptides from Pinto beans (Phaseolus vulgaris cv. Pinto). Food Chem 190:331–337

    Article  CAS  Google Scholar 

  • Oberley L (1988) Free radicals and diabetes. Free Radic Bio Med 5:113–124

    Article  CAS  Google Scholar 

  • Pinar SG, Pezik E, Agardan BM, Celebi N (2021) Development of cyclosporine A nanosuspension: cytotoxicity and permeability on Caco-2 cell lines. Pharm Dev Technol 27:52–62

    Article  Google Scholar 

  • Qiao Q, Xue W, Feng Z (2018) Variability of seed oil content, fatty acid composition, and nervonic acid content in Acer truncatum, native to 14 regions of China. Grasas Aceites 69(4):e274

    Article  CAS  Google Scholar 

  • Qiao HR, Bi XJ, Zhang YY, Liu MR, Zu SC, Jia N, Jiang SG, Lu Q, Zu YG, Bao YH (2020) Enzymic polypeptide antioxidant activity and inhibitory activity on α-glucosidase and α-amylase from Paeonia ostii cake. Ind Crop Prod 146:112158

    Article  CAS  Google Scholar 

  • Qin GW (2003) Biological characteristics and economic value of Metasequoia. For Shanxi 5:28

    Google Scholar 

  • Radeva G, Valchev I, Petrin S, Valcheva E, Tsekova P (2012) Kinetic model of enzymatic hydrolysis of steam-exploded wheat straw. Carbohydr Polym 87:1280–1285

    Article  CAS  Google Scholar 

  • Rodriguez M, Garcia Filleria SF, Tironi VA (2020) Simulated gastrointestinal digestion of amaranth flour and protein isolate: comparison of methodologies and release of antioxidant peptides. Food Res Int 138:109735

    Article  CAS  Google Scholar 

  • Rossing P, de Zeeuw D (2011) Need for better diabetes treatment for improved renal outcome. Kidney Int 79:S28–S32

    Article  Google Scholar 

  • Ryu HW, Cho J, Curtis-Long M, Yuk HJ, Kim YS, Jung S, Kim Y, Lee B, Park K (2011) H: α-Glucosidase inhibition and antihyperglycemic activity of prenylated xanthones from Garcinia mangostana. Phytochemistry 72:2148–2154

    Article  CAS  Google Scholar 

  • Saidi S, Deratani A, Belleville MP, Amar RB (2014) Antioxidant properties of peptide fractions from tuna dark muscle protein by-product hydrolysate produced by membrane fractionation process. Food Res Int 65:329–336

    Article  CAS  Google Scholar 

  • Sanchez-Silva L, Lopez-Gonzalez D, Villasenor J, Sanchez P, Valverde JL (2012) Thermogravimetric-mass spectrometric analysis of lignocellulosic and marine biomass pyrolysis. Bioresource Technol 109:163–172

    Article  CAS  Google Scholar 

  • Siow H, Gan C (2016) Optimization study in extracting anti-oxidative and α-amylase inhibitor peptides from cumin seeds (Cuminum Cyminum): bioactive peptides from Cuminum Cyminum. J Food Biochem 41:12280–12292

    Article  Google Scholar 

  • Siow H, Lim T, Gan C (2016) Development of a workflow for screening and identification of α-amylase inhibitory peptides from food source using an integrated Bioinformatics-phage display approach: case study-Cumin seed. Food Chem 214:67–76

    Article  Google Scholar 

  • Stojkovic D, Smiljkovic M, Ciric A, Glamoclija J, Van Griensven L, Ferreira ICFR, Sokovic M (2019) An insight into antidiabetic properties of six medicinal and edible mushrooms: inhibition of α-amylase and α-glucosidase linked to type-2 diabetes. S Afr J Bot 120:100–103

    Article  CAS  Google Scholar 

  • Tepe AS, Ozaslan M (2020) Anti-Alzheimer, anti-diabetic, skin-whitening, and antioxidant activities of the essential oil of Cinnamomum zeylanicum. Ind Crop Prod 145:112069

    Article  Google Scholar 

  • Thamnarathip P, Jangchud K, Nitisinprasert S, Vardhanabhuti B (2016) Identification of peptide molecular weight from rice bran protein hydrolysate with high antioxidant activity. J Cereal Sci 69:329–335

    Article  CAS  Google Scholar 

  • Torkova A, Ryazantseva K, Agarkova E, Kruchinin A, Tsentalovich M, Fedorova T (2017) Rational design of enzyme compositions for the production of functional hydrolysates of cow milk whey proteins. Appl Biochem Microbiol 53:669–679

    Article  CAS  Google Scholar 

  • Vanvi A, Tsopmo A (2016) Pepsin digested oat bran proteins: separation, antioxidant activity, and identification of new peptides. J Chem 2016:1–8

    Article  Google Scholar 

  • Wang XY, Li YJ, Wang SQ (2007) A new resource of food protein: Acer truncatum Bunge protein. China Oils Fats 32(8):30–33

    Google Scholar 

  • Wang L, Liang Q, Chen T, Wang Z, Xu J, Ma H (2014) Characterization of collagen from the skin of Amur sturgeon (Acipenser schrenckii). Food Hydrocoll 38:104–109

    Article  Google Scholar 

  • West IC (2000) Radicals and oxidative stress in diabetes. Diabetic Med 17:171–180

    Article  CAS  Google Scholar 

  • FU WHO (2007) Protein amino acid requirements in human nutrition. WHO Tech Rep Ser 935:1–265

    Google Scholar 

  • Wittner N, Slezsak J, Broos W, Geerts J, Gergely S, Vlaeminck SE, Cornet I (2022) Rapid lignin quantification for fungal wood pretreatment by ATR-FTIR spectroscopy. Spectrochim Acta A 285:121912

    Article  Google Scholar 

  • Yu Z, Yin Y, Zhao W, Yu Y, Liu B, Liu J, Chen F (2011) Novel peptides derived from egg white protein inhibiting alpha-glucosidase. Food Chem 129:1376–1382

    Article  CAS  Google Scholar 

  • Yu ZP, Yin YG, Zhao WZ, Liu JB, Chen F (2012) Anti-diabetic activity peptides from albumin against α-glucosidase and α-amylase. Food Chem 135:2078–2085

    Article  CAS  Google Scholar 

  • Zhang L, Tu ZC, Xie X, Lu Y, Wang ZX, Wang H, Sha XM (2016) Antihyperglycemic, antioxidant activities of two Acer palmatum cultivars, and identification of phenolics profile by UPLC-QTOF-MS/MS: new natural sources of functional constituents. Ind Crop Prod 89:522–532

    Article  CAS  Google Scholar 

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Funding

This work was financially supported by the Heilongjiang Science Fund Project (LH2022C005); Heilongjiang Major Science and Technology Projects (2020ZX07B01-3).

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Author notes

  1. Liang Wei and Hui Li contributed equally to this work as co-first authors.

  2. Qi Lu and Yihong Bao are co-corresponding authors.

Authors and Affiliations

  1. College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin, 150040, China

    Liang Wei, Hui Li, Huiying Yang, Qi Lu & Yuangang Zu

  2. School of Forestry, Food Science and Engineering, Northeast Forestry University, Harbin, 150040, China

    Xinxin Yu, Duowen Pu & Yihong Bao

  3. Harbin Vocational & Technical College, Harbin, 150040, China

    Minghua Zhu

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  1. Liang Wei
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Contributions

Author’s contributions are as follows: QL, YZ and LW conceived, designed the study and wrote manuscript. XY performed the preparation of raw materials, process optimization. HY, MZ and DP evaluated these samples. YB and LW processed and analyzed the experimental data and plotted them. HL and LW wrote the revised manuscript.

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Correspondence to Qi Lu or Yihong Bao.

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Wei, L., Li, H., Yu, X. et al. Amino Acid Composition, Antioxidant, α-Glucosidase and α-Amylase Inhibitory Activities of the Enzymic Polypeptide from Acer truncatum Seed Meal. Int J Pept Res Ther 29, 4 (2023). https://doi.org/10.1007/s10989-022-10473-3

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