Abstract
In this study, we aimed to determine the potential functional properties and biological activities of the hydrolysates of perilla seed meal (PSM), which is a by-product of perilla seed oil extraction. PSM protein was hydrolyzed independently by using five proteases, and their functional and biological properties were analyzed. PSM protein hydrolysate exhibited high solubility at most of the tested pH values, and the trypsin-treated hydrolysate exhibited the highest water and oil absorption capacity. The neutrase-treated hydrolysate was most effective in scavenging the 1,1-diphenyl-2-picrylhydrazine radicals, whereas the pepsin-treated hydrolysate showed the highest angiotensin I-converting enzyme inhibitory effect, and anti-inflammatory activity. Trypsin-treated hydrolysate exhibited the highest scavenging activity against of 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid radicals with the IC50 of 109.72 µg/mL. The results of the present study suggest that the type of protease used for the treatment significantly influences the functional properties and biological activities of the resulting PSM protein hydrolysates.
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Ahn C, Cho Y, Je J. Purification and anti-inflammatory action of tripeptide from salmon pectoral fin byproduct protein hydrolysate. Food Chem. 168: 151-156 (2015)
Ao J, Li B. Stability and antioxidative activities of casein peptide fractions during simulated gastrointestinal digestion in vitro: Charge properties of peptides affect digestive stability. Food Res. Int. 52: 334-341 (2013)
Cao W, Zhang C, Hong P, Ji H, Hao J. Purification and identification of an ACE inhibitory peptide from the peptic hydrolysate of Acetes chinensis and its antihypertensive effects in spontaneously hypertensive rats. Int. J. Food Sci. Technol. 45: 959-965 (2010)
Chen M, Li B. The effect of molecular weights on the survivability of casein-derived antioxidant peptides after the simulated gastrointestinal digestion. Innov. Food Sci. Emerg. Technol. 16: 341-348 (2012)
da Silva CM, Zanqui AB, Gohara AK, de Souza, Aloísio Henrique Pereira, Cardozo-Filho L, Visentainer JV, Chiavelli LUR, Bittencourt PRS, da Silva EA, Matsushita M. Compressed n-propane extraction of lipids and bioactive compounds from perilla (Perilla frutescens). J. Supercrit. Fluids 102: 1-8 (2015)
Du M, Xie J, Gong B, Xu X, Tang W, Li X, Li C, Xie M. Extraction, physicochemical characteristics and functional properties of mung bean protein. Food Hydrocoll. 76: 131-140 (2018)
Guan X, Yao H, Chen Z, Shan L, Zhang M. Some functional properties of oat bran protein concentrate modified by trypsin. Food Chem. 101: 163-170 (2007)
Jamdar S, Rajalakshmi V, Pednekar M, Juan F, Yardi V, Sharma A. Influence of degree of hydrolysis on functional properties, antioxidant activity and ACE inhibitory activity of peanut protein hydrolysate. Food Chem. 121: 178-184 (2010)
Jang HL, Liceaga AM, Yoon KY. Isolation and characteristics of anti-inflammatory peptides from enzymatic hydrolysates of sandfish (Arctoscopus japonicus) protein. J. Aquat. Food Prod. T. 26: 234-244 (2017)
Je J, Park SY, Hwang J, Ahn C. Amino acid composition and in vitro antioxidant and cytoprotective activity of abalone viscera hydrolysate. J. Funct. Foods 16: 94-103 (2015)
Kim JM, Liceaga AM, Yoon KY. Purification and identification of an antioxidant peptide from perilla seed (Perilla frutescens) meal protein hydrolysate. Food Sci. Nutr. 7: 1645-1655 (2019)
Kong X, Zhou H, Qian H. Enzymatic hydrolysis of wheat gluten by proteases and properties of the resulting hydrolysates. Food Chem. 102: 759-763 (2007)
Liu B, Chiang P. Production of hydrolysate with antioxidative activity and functional properties by enzymatic hydrolysis of defatted sesame (Sesamum indicum L.). Int. J. Appl. Sci. Eng. 6: 73-83 (2008)
Longvah T, Deosthale Y. Effect of dehulling, cooking and roasting on the protein quality of Perilla frutescens seed. Food Chem. 63: 519-523 (1998)
Meram C, Wu J. Anti-inflammatory effects of egg yolk livetins (α, β, and γ-livetin) fraction and its enzymatic hydrolysates in lipopolysaccharide-induced RAW 264.7 macrophages. Food Res. Int. 100: 449-459 (2017)
Nguyen E, Jones O, Kim YHB, San Martin-Gonzalez F, Liceaga AM. Impact of microwave-assisted enzymatic hydrolysis on functional and antioxidant properties of rainbow trout Oncorhynchus mykiss by-products. Fish. Sci. 83: 317-331 (2017)
Oita S, Kimura T, Shibuya Y, Nihei N, Tanahashi K. Extraction and digestibility of Perilla frutescens seed protein. Jpn. Agric. Res. Q. 42: 211-214 (2008)
Park BY, Yoon KY. Biological activity of enzymatic hydrolysates and the membrane ultrafiltration fractions from perilla seed meal protein. Czech J. Food Sci. 37: 180-185 (2019a)
Park BY, Yoon KY. Functional properties of enzymatic hydrolysate and peptide fractions from perilla seed meal protein. Pol. J. Food Nutr. Sci. 2: 119-127 (2019b)
Periago MJ, Vidal ML, Ros G, Rincón F, Martínez C, López G, Rodrigo J, Martínez I. Influence of enzymatic treatment on the nutritional and functional properties of pea flour. Food Chem. 63: 71-78 (1998)
Phongthai S, Lim S, Rawdkuen S. Optimization of microwave-assisted extraction of rice bran protein and its hydrolysates properties. J. Cereal Sci. 70: 146-154 (2016)
Picariello G, Ferranti P, Fierro O, Mamone G, Caira S, Di Luccia A, Monica S, Addeo F. Peptides surviving the simulated gastrointestinal digestion of milk proteins: Biological and toxicological implications. J. Chromatogr. B Analyt. Technol. Biomed Life Sci. 878: 295-308 (2010)
Rajapakse N, Mendis E, Byun H, Kim S. Purification and in vitro antioxidative effects of giant squid muscle peptides on free radical-mediated oxidative systems. J. Nutr. Biochem. 16: 562-569 (2005)
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26: 1231-1237 (1999)
Sheih I, Fang TJ, Wu T. Isolation and characterisation of a novel angiotensin I-converting enzyme (ACE) inhibitory peptide from the algae protein waste. Food Chem. 115: 279-284 (2009)
Tan E, Ying-Yuan N, Gan C. A comparative study of physicochemical characteristics and functionalities of pinto bean protein isolate (PBPI) against the soybean protein isolate (SPI) after the extraction optimisation. Food Chem. 152: 447-455 (2014)
Wang B, Xie N, Li B. Influence of peptide characteristics on their stability, intestinal transport, and in vitro bioavailability: A review. J. Food Biochem. 43: e12571 (2019)
Xie J, Du M, Shen M, Wu T, Lin L. Physico-chemical properties, antioxidant activities and angiotensin-I converting enzyme inhibitory of protein hydrolysates from mung bean (Vigna radiate). Food Chem. 270: 243-250 (2019)
Yan M, Tao H, Qin S. Effect of enzyme type on the antioxidant activities and functional properties of enzymatic hydrolysates from sea cucumber (Cucumaria frondosa) viscera. J. Aquat. Food Prod. Technol. 25: 940-952 (2016)
Yang J, Hu L, Cai T, Chen Q, Ma Q, Yang J, Meng C, Hong J. Purification and identification of two novel antioxidant peptides from perilla (Perilla frutescens L. Britton) seed protein hydrolysates. PloS One. 13: e0200021 (2018)
Yim M, Lee J. Functional properties of fractionated soy protein isolates by proteases from Meju. Food Sci. Biotechnol. 9: 253-257 (2000)
You L, Zhao M, Regenstein JM, Ren J. Purification and identification of antioxidative peptides from loach (Misgurnus anguillicaudatus) protein hydrolysate by consecutive chromatography and electrospray ionization-mass spectrometry. Food Res. Int. 43: 1167-1173 (2010)
Zhu J, Fu Q. Optimization of ultrasound-assisted extraction process of perilla seed meal proteins. Food Sci. Biotechnol. 21: 1701-1706 (2012)
Zhu K, Zhou H, Qian H. Antioxidant and free radical-scavenging activities of wheat germ protein hydrolysates (WGPH) prepared with alcalase. Process Biochem. 41: 1296-1302 (2006)
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This work was supported by the Yeungnam University Research Grant.
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Kim, J.M., Yoon, K.Y. Functional properties and biological activities of perilla seed meal protein hydrolysates obtained by using different proteolytic enzymes. Food Sci Biotechnol 29, 1553–1562 (2020). https://doi.org/10.1007/s10068-020-00810-x
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DOI: https://doi.org/10.1007/s10068-020-00810-x