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Isolation, Identification, and Characterization of a Novel Bacteriocin Produced by Brevibacillus laterosporus DS-3 Against Methicillin-Resistant Staphylococcus aureus (MRSA)

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Abstract

Isolation and characterization of a bacteriocin from Brevibacillus laterosporus DS-3 and its ability in producing antibacterial activity were examined in this study. We isolated 48 bacterial strains from different native ecological niches. Sources included lake water, soil, air, and food. Among the isolates from the soil sample, morphological and biochemical tests identified Brev. laterosporus DS-3, which showed great potential for bacteriocin production. It was purified by ammonium sulfate precipitation, then applied in a Sephadex G-15 gel filtration column connected to a purification of AKTA Pure (USA) and reverse phase chromatography. MALDI-TOF mass spectrometry indicated that bacteriocin DS-3 has a low molecular weight of 1592.579 Da. LNTLETEEWFFK was identified as the sequence by mass spectrometry analysis in tandem liquid chromatography and MASCOT research. This bacteriocin inhibited Gram-positive and Gram-negative bacteria and was effective against Methicillin-resistant Staphylococcus aureus (MRSA). Brev. laterosporus DS-3 present in here is thermostable and tolerant to extreme pH. However, inactivation of its antibacterial activity was observed when treated with papain and trypsin but not pepsin, proteinase K, and catalase.

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Funding

This study was funded by National Key Research and Development Plan (Grant Number: 2016YFD0501301); National Natural Science Foundation of China (Grant Numbers: 31572574, 31872519, 31702293); Natural Science Foundation of Jilin Province (Grant Number: 20190201294JC). Theme Guidance Project of Department of Science and Technology of Jilin Province (Grant Number: 20180101003JC).

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Authors and Affiliations

  1. Department of Basic Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, Jilin, China

    Kokou Ayefounin Odah, Wen-Long Dong, Liu Lei, Luke Atiewin Atiah, Yi-ming Wang, Ling-Cong Kong & Hong-Xia Ma

  2. The Key Laboratory of Animal Production, Product Quality, and Security, Ministry of Education, Jilin Agricultural University, Changchun, Jilin, China

    Hong-Xia Ma

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  1. Kokou Ayefounin Odah
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  2. Wen-Long Dong
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  3. Liu Lei
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  4. Luke Atiewin Atiah
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  5. Yi-ming Wang
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  6. Ling-Cong Kong
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  7. Hong-Xia Ma
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Correspondence to Ling-Cong Kong or Hong-Xia Ma.

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Odah, K.A., Dong, WL., Lei, L. et al. Isolation, Identification, and Characterization of a Novel Bacteriocin Produced by Brevibacillus laterosporus DS-3 Against Methicillin-Resistant Staphylococcus aureus (MRSA). Int J Pept Res Ther 26, 709–715 (2020). https://doi.org/10.1007/s10989-019-09878-4

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