Abstract
Bacteriocins are natural antimicrobial peptides with attractive possible applications in food preservation and health care. In the present study, bacteriocin producing bacterial strain Pseudomonas aeruginosa were isolated from soil which exhibited antagonistic activity against Methicillin Resistant Staphylococcus aureus (MRSA) bacteria. The bacteriocin producing strain TA6 was confirmed as P. aeruginosa by biochemical tests and 16S rRNA gene sequence analysis. Maximum bacteriocin activity (100 AU ml−1) was observed at 37 °C with pH 6.0 in 24 h time duration. SDS–PAGE analysis of the extracellular protein of P. aeruginosa TA6 revealed a bacteriocin-like protein with a molecular mass of ~10 kDa. MRSA cells were treated with culture supernatant of P. aeruginosa TA6 and analyzed by FT-IR. The treated and untreated MRSA showed band variations at 671 and 3460 cm−1 corresponding to alkyl and amide group respectively. Mixed proportions of dead and live control populations were analyzed by flow cytometry to determine detection limits of the Dead/Live cells. The flow cytometry detection of defined proportions of dead (p2) and live (p1) cells at 3 h were p2 = 60.5%; p1 = 39.5% and 6 h p2 = 66.5%; p1 = 33.5% respectively. The scanning electron microscopy observation showed the main changes in the cell membrane structural integrity of S. aureus after exposure to the bacteriocin from P. aeruginosa TA6 at 12 h incubation. Together, the results suggested that bacteriocin from P. aeruginosa TA6 was effective against MRSA.
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References
Baggett HC, Hennessy TW, Leman R, Hamlin C, Bruden D, Reasonover A (2003) An outbreak of community-onset methicillin-resistant Staphylococcus aureus skin infections in southwestern Alaska. Infect Control Hosp Epidemiol 24:397–402
Bartoloni A, Bartalesi F, Mantella A, Dell’Amico E, Roselli M, Strohmeyer M (2004) High prevalence of acquired antimicrobial resistance unrelated to heavy antimicrobial consumption. J Infect Dis 189:1291–1294
Bhunia AK, Johnson MC, Ray B (1987) Direct detection of an antimicrobial peptide of Pediococcus acidilactici in sodium dodecyl sulphate-polyacrylamide gel electrophoresis. J Ind Microbiol 2:319–322
Brotz H, Josten M, Wiedemann I, Schneider U, Gotz F, Bierbaum G (1998) Role of lipid-bound peptidoglycan precursors in the formation of pores by nisin, epidermin and other lantibiotics. Mol Microbiol 30:317–327
Church D, Elsayed S, Reid O, Winston B, Lindsay R (2006) Burn wound infections. Clin Microbiol Rev 19:403–434
Cosgrove SE, Carmeli Y (2003) The impact of antimicrobial resistance on health and economic outcomes. Clin Infect Dis 36:1433–1437
Cotter PD, Hill C, Ross RP (2005) Bacteriocins: developing innate immunity for food. Nature Rev Microbiol 3:777–788
Daum RS (2007) Skin and soft-tissue infections caused by methicillinresistant Staphylococcus aureus. N Engl J Med 357:380–390
de Jong A, van Hijum SAFT., Bijlsma JJE, Kok J, Kuipers OP (2006) BAGEL: a web-based bacteriocin genome mining tool. Nucleic Acids Res 34:273–279
Fialkov JA, Holy C, Forrest CR, Phillip JH, Antonyshyn OM (2001) Postoperative infections in craniofacial reconstructive procedures. J Craniofac Surg 12:362–368
Fridkin SK, Hageman JC, Morrison M, Sanza LT, Como Sabetti K, Jernigan JA (2005) Methicillin-resistant Staphylococcus aureus disease in three communities. N Engl J Med 352:1436–1444
Gratia A (1925) Sur un remarquable exemple d’antagonisme entre deux souches de coilbacille. Comp Rend Soc Biol 93:1040–1041
Heng NCK, Wescombe PA, Burton JP, Jack RW, Tagg JR (2007) The diversity of bacteriocins in Gram-positive bacteria. In: Riley MA, Chavan M (eds) Bacteriocins: ecology and evolution. Springer, Berlin, pp 45–92
Kirkup BC (2006) Bacteriocins as oral and gastrointestinal antibiotics: theoretical considerations, applied research, and practical applications. Curr Med Chem 13:335–3350
Krishnan V, Johnson JV, Helfrick JF (1993) Management of maxillofacial infections: a review of 50 cases. J Oral Maxillofac Surg 51:868–873
Kumar S, Tamura K, Nei M (2004) Mega3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163
Mathur H, Field D, Rea MC, Cotter PD, Hill C, Ross RP (2017) Bacteriocin-antimicrobial synergy: a medical and food perspective. Front Microbiol 8:1205
McDougall PP, Shane S, Oviatt BM (1994) Explaining the formation of international new ventures: the limits of theories from international business research. J Bus Ventur 9(6):469–487
Meyer J, Rogne P, Opegard C, Haugen H, Kristiansen P (2009) Structure-function relationships of the non-lanthionine-containing peptide (class II) bacteriocins produced by gram-positive bacteria. Curr Pharm Biotechnol 10:19–37
Motta AS, Brandelli A (2008) Evaluation of environmental conditions for production of bacteriocin-like substance by Bacillus sp. strain P34. World J Micro Biotech 24:641–646
Naz SA, Jabeen N, Sohail M, Rasool SA (2015) Biophysicochemical characterization of Pyocin SA 189 Produced by Pseudomonas aeroginosa SA189. Braz J Micro 46 (4):1147–1154
Okesola AO (2011) Community-acquired methicillin-resistant Staphylococcus aureus - a review of literature. Afr J Med Sci 40:97–107
Otto M (2008) Staphylococcal biofilms. Curr Top Microbiol Immunol 322:207–228
Padilla C, Lobos O, Brevis P (2002) Effect of the bacteriocin PsVP- 10 produced by Pseudomonas sp. On sensitive bacterial strains. De Microbiologia 44:19–23
Papagianni M (2003) Ribosomally synthesized peptides with antimicrobial properties: biosynthesis, structure, function, and applications. Biotech Adv 21:465–499
Piper C, Hill C, Cotter PD, Ross RP (2011) Bioengineering of a nisin A-producing Lactococcus lactis to create isogenic strains producing the natural variants nisin F, Q, and Z. Microb Biotech 4:375–382
Riley MA, Wertz JE (2002) Bacteriocins: evolution, ecology, and application. Annu Rev Microbiol 56:117–137
Rubiee R, Mudhaffar S, Hassan F (1988) Purification and characterization of pyocins from Pseudomonas aeroginosa. Folia Microbiol 30:25–29
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Saleem F, Ahmed S, Yaqoob Z (2009) Comparative study of two bacteriocins produced by representative indigenous soil bacteria. Pak J Pharma Sci 22:252–258
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Nova York
Sano Y, Kageyama M (1981) Purification and properties of an S-type pyocin, Pyocin AP41. J Bacteriol 146:733–739
Schagger H, von Jagow G (1987) Tricine-sodium dodecyl sulfate polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166:368–379
Severina E, Severin A, Tomasz A (1998) Antibacterial efficacy of nisin against multidrug-resistant Gram-positive pathogens. J Antimicrob Chemother 41:341–347
Shand RF, Leyva KJ (2008) Archaeal antimicrobials: an undiscovered country. In: Blum P (ed) Archaea: new models for prokaryotic biology. Caister Academic, Norfolk, pp 233–242
Tagg JR, Dajani AS, Wannamaker LW (1976) Bacteriocins of Gram-positive bacteria. Bacteriol Rev 40:722–756
Toba T, Yoshioka E, Itoh T (1991) Potential of Lactobacillus gasseri isolated from infant faeces to produce bacteriocin. Lett Appl Microbio 12:228–231
Vindenes H, Bjerknes R (1995) Microbial colonization of large wounds. Burns 21:575–579
Williams I, Paul F, Lloyd D, Jepras R, Critchley I, Newman M (1999) Flow cytometry and other techniques show that Staphylococcus aureus undergoes significant physiological changes in the early stages of surface-attached culture. Microbiology 145:1325–1333
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The authors gratefully acknowledge the Department of Science and Technology, New Delhi for providing financial supports under DST-WOS-A start of grant for (DST/SR/WOS- A/LS-629/2012(G)), Scheme.
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Arumugam, T., Dhanam, S., Rameshkumar, N. et al. Inhibition of Methicillin Resistant Staphylococcus aureus by Bacteriocin Producing Pseudomonas aeruginosa. Int J Pept Res Ther 25, 339–348 (2019). https://doi.org/10.1007/s10989-018-9676-y
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DOI: https://doi.org/10.1007/s10989-018-9676-y