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Substarte Specificty and Immobilization Studies of Purified Solanain from the Latex of Vallaris solanacea

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Abstract

A novel cysteine protease has been purified to electrophoretic homogeneity from apocyanacean member Vallaris solanacea. Molecular weight of the solanain was determined as 28.5 kDa using sodium dodecyl sulphate polyacrylamide gel electrophoresis. Purified protease was named solanain and it was further characterized. An internal tryptic fragment was identified by MALDI TOF, and this peptide showed a homology (66% sequence identity) with the target sequence found as cysteine endopeptidase from Ricinus communis. The purpose of the present work is to examine the specificity towards synthetic peptide and ester substrates and also to compare with other cysteine proteases. In the present work protease solanain from V. solanacea was immobilized using various matrices such as calcium alginate, polyacrylamide, κ-carrageenan, chitosan and amberlite MB-150 employing entrapment and adsorption techniques. The purified solanain from the latex of V. solanacea exhibited broad specificity. Like other cysteine proteases it showed peptidase and amidase activity. However considerable difference was noticed in the rate of hydrolysis and also exhibited difference in specificity towards simple peptide substrates. Immobilization of solanain on amberlite MB-150 beads enhanced the enzyme stability against changes of pH and temperature. The immobilized enzyme retained 95, 89, 84 and 80% activity at the end of 2nd, 3rd, 4th and 5th cycle respectively which indicates that immobilized enzyme can be reused for 5 cycles.

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Abbreviations

β-ME:

β-Mercapto ethanol

TCA:

Tri chloro acetate

EDTA:

Ethylene diamino tetra acetic acid

PCMB:

p-Chloromercuribenzoate

SDS:

Sodium dodecyl sulphate

DEAE:

Diethyl amino ethyl

PMSF:

Phenyl methyl sulfonyl fluoride

DEAE:

Diethyl aminoethyl

TLC:

Thin layer chromatography

EDTA:

Ethylene diamino tetra acetic acid

CBZ:

Carbo benzyloxy

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Acknowledgements

We thank the Department of Biochemistry, Gandhi Institute of Technology and Management, for providing the necessary facilities to conduct our research.

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Authors and Affiliations

  1. Department of Food Technology, Vikrama Simhapuri University, SPSR, Nellore, Andhra Pradesh, 524003, India

    Silpa Somavarapu

  2. Department of Biotechnology, Centre for Bioprocess Technology, K L E F University, Guntur, Andhra Pradesh, India

    Sandeep Vemula

  3. Department of Biochemistry, Gitam University, Visakhapatnam, Andhra Pradesh, India

    I. Bhaskar Reddy

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  1. Silpa Somavarapu
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  2. Sandeep Vemula
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  3. I. Bhaskar Reddy
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Correspondence to Silpa Somavarapu.

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The article is entirely a study on plants. It does not include any animals or human participants.

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Somavarapu, S., Vemula, S. & Bhaskar Reddy, I. Substarte Specificty and Immobilization Studies of Purified Solanain from the Latex of Vallaris solanacea. Int J Pept Res Ther 25, 169–180 (2019). https://doi.org/10.1007/s10989-017-9659-4

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