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Isolation, Purification and Partial Characterization of Crotalaria pallida Aiton Seed Proteins

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Abstract

Crotalaria pallida Aiton (Smooth rattlebox in English) is widely and wildly distributed in India. The seed of this plant is a valuable source of non-traditional proteins (about 22%) but attention was never been paid to explore the protein content. Chemical investigation has been conducted on Crotalaria pallida seeds (de-oiled) and proteins have been extracted in aqueous solution with different pHs or various concentrations of NaCl, KCl, Na2SO3 and CaCl2·2H2O at pH 7.0. The present study includes isolation, purification, and fractionation of seed protein along with its amino acid composition, molecular weight determination and surface topographies. Multiple polypeptide bands have been identified in the range of 16.5–61.6 kDa. The overall study confirms the seeds of this plant as an important source of unexplored protein.

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Acknowledgements

Authors are indebted to the University of Burdwan, Burdwan, West Bengal, DSA, UGC, New Delhi, India as well as Department of Chemistry, The University of Texas-Rio Grande Valley and the Kleberg Foundation of Texas, USA.

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Authors and Affiliations

  1. Natural Product Laboratory, Department of Chemistry, The University of Burdwan, Burdwan, 713104, West Bengal, India

    Sushobhan Ukil & Subrata Laskar

  2. Department of Chemistry, The University of Texas-Rio Grande Valley, 1201 West University Drive, Edinburg, Texas, 78539, USA

    Debasish Bandyopadhyay

Authors
  1. Sushobhan Ukil
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  2. Subrata Laskar
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  3. Debasish Bandyopadhyay
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Correspondence to Subrata Laskar or Debasish Bandyopadhyay.

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Ukil, S., Laskar, S. & Bandyopadhyay, D. Isolation, Purification and Partial Characterization of Crotalaria pallida Aiton Seed Proteins. Int J Pept Res Ther 23, 461–467 (2017). https://doi.org/10.1007/s10989-017-9578-4

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  • DOI: https://doi.org/10.1007/s10989-017-9578-4

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