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ACE Inhibitory and Antioxidant Activities of Novel Peptides from Scorpaena notata By-product Protein Hydrolysate

International Journal of Peptide Research and Therapeutics volume 23, pages 13–23 (2017)Cite this article

Abstract

This study describes the isolation of angiotensin I converting enzyme and antioxidative peptides from head protein hydrolysate of red scorpionfish (Scorpaena notata) prepared by treatment with a protease from the fungus Penicillium digitatum. After ultrafiltration, three peptides were isolated by a two-step procedure: size exclusion chromatography on a Toyopearl HW-40 followed by reversed-phase high performance liquid chromatography (RP-HPLC) with a high purification yield of 2.22 mg of peptide/g of initial protein. Active peptides were then identified by nanoscale liquid chromatography coupled to tandem mass spectrometry (nanoLC/MS–MS), corresponding to the following sequences: Gln–Gln–Pro–His–Ser–Arg–Ser–Lys–Gly–Phe–Pro–Gly–Pro (1424.724 Da), Gly–Gln–Lys–Ser–Val–Pro–Glu–Val–Arg (1000.565 Da) and Val–Glu–Gly–Lys–Ser–Pro–Asn–Val (830.448 Da). Peptides D-I, E-I and F-I showed high angiotensin-I converting enzyme inhibitory activity with an IC50 values of 0.98, 1.69 and 1.44 µM, respectively as well as a synergistic antioxidant activity between the different fractions. Thus, we have demonstrated that underutilized wastes can be valorized by production of peptides that can be used as potential therapeutic compounds active against oxidative stress and hypertension.

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Acknowledgments

This work was supported by the financial project of LIP-MB Laboratory, INSAT, Carthage University, Ministry of Higher Education and Scientific Research of Tunisia.

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    Authors and Affiliations

    1. Laboratory of Protein Engineering and Bioactive Molecules (LIP-MB), National Institute of Applied Sciences and Technology, University of Carthage, B. P. 676, 1080, Tunis Cedex, Tunisia

      Neyssene Aissaoui, Ferid Abidi & M. Nejib Marzouki

    2. CNRS UMR 6270, Laboratory of Polymers, Biopolymers and Surfaces, University of Rouen, Mont-Saint-Aignan, France

      Julie Hardouin & Thierry Jouenne

    3. Laboratory of Venoms and Therapeutic Biomolecules, Institut Pasteur of Tunis, Tunis, Tunisia

      Zaineb Abdelkafi & Naziha Marrakchi

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    1. Neyssene Aissaoui
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    2. Ferid Abidi
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    4. Zaineb Abdelkafi
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    5. Naziha Marrakchi
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    7. M. Nejib Marzouki
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    Correspondence to Neyssene Aissaoui.

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    This manuscript does not contain any studies with human subjects performed by any of authors.

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    Ferid Abidi and Julie Hardouin are the authors contributed equally to this work.

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    Aissaoui, N., Abidi, F., Hardouin, J. et al. ACE Inhibitory and Antioxidant Activities of Novel Peptides from Scorpaena notata By-product Protein Hydrolysate. Int J Pept Res Ther 23, 13–23 (2017). https://doi.org/10.1007/s10989-016-9536-6

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    • DOI: https://doi.org/10.1007/s10989-016-9536-6

    Keywords

    • Angiotensin-I converting enzyme
    • Antioxidant peptides
    • Protein hydrolysate
    • Scorpaena notata