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From Inducing Autophagy to Programmed Cell Death? The PI3K Functional Domain Study of Protein Latcripin-1 from Lentinula edodes C91-3

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Abstract

The functional study of the edible mycoprotein is important for elucidating the efficacy mechanism of fungi. There are more broad prospects on the field of antitumor mycoprotein. The expression map of Lentinula edodes C91-3 was sequenced and analyzed on the previous work. A novel antitumor mycoprotein—Latcripin-1 (LP1) was found at the first time. In order to clarify the mechanism of its inducing programmed cell death in lung cancer cells, protein LP1 PI3K (phosphatidylinositol 3-kinase) functional domain peptide was obtained by the Rosetta gami prokaryotic expression and purification system. Western blot was used to identify the functional domain and the autophagosomal marker—microtubule associated protein light chain 3. Transmission electron microscopy technology was used to analysed the ultrastructure of the treated cell. Flow cytometry and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) methods were used to detect the biological functions of domain PI3K to lung cancer cell. This work demonstrates that the functional domain PI3K of LP1 maybe induces lung cancer A549 to programmed cell death through autophagy pathway. The functional domain PI3K in L. edodes C91-3 should be ascribed to class III PI3K. Though further study is needed, it will shed new light into developing new anti-tumor polypeptide drugs.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Microbiology, Dalian Medical University, No. 9 West Section, Lvshun South Road, Lvshunkou District, Dalian, 116044, Liaoning, China

    Ben Liu, Siruo Zhang, Mintao Zhong, Li Sha, Yongzhi Lun, Wei Zhang, Xiaoli Wang, Xingyun Li, Jing Cao, Anhong Ning & Min Huang

Authors
  1. Ben Liu
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  2. Siruo Zhang
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  3. Mintao Zhong
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  4. Li Sha
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  5. Yongzhi Lun
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  6. Wei Zhang
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  7. Xiaoli Wang
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  8. Xingyun Li
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  9. Jing Cao
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  10. Anhong Ning
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  11. Min Huang
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Correspondence to Min Huang.

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Liu, B., Zhang, S., Zhong, M. et al. From Inducing Autophagy to Programmed Cell Death? The PI3K Functional Domain Study of Protein Latcripin-1 from Lentinula edodes C91-3. Int J Pept Res Ther 20, 341–352 (2014). https://doi.org/10.1007/s10989-014-9399-7

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  • DOI: https://doi.org/10.1007/s10989-014-9399-7

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