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Immunohistochemical Study of Leucine-Enkephalin and Delta Opioid Receptor in Mantles and Feet of the Octopus Octopus ocellatus Gray

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Abstract

The study was supposed to determine whether leucine-enkephalin (leu-Enk) or delta opioid receptor was presented in mantles and feet of the Octopus Octopus ocellatus. The results showed that they were found in the mantles, wrists, interbrachial membrane, and funnels of the Octopus Octopus ocellatus. The immunoreactivities of leu-Enk and delta opioid receptor were observed in the epithelial cells of dorsal mantle, wrist and interbrachial membrane. Strong one was presented in both the connective tissue which was close to the epithelium of dorsal mantle, wrist and interbrachial membrane and a small amount of connective tissue cells and nerve fibers, while less positive in other parts. A little of immunoreactive material was also observed in the ventral mantle and funnel, for example, weak one was in the epithelial tissue, the adjacent cells and nerve fibers. The different densities of leu-Enk and delta opioid receptor in mantles and feet of the Octopus O. ocellatus may be related to the different functions.

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Abbreviations

Enk:

Enkephalin

Met-Enk:

Methionine-enkephalin

Leu-Enk:

Leucine-enkephalin

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Acknowledgments

This work was supported by a grant from the “973” Project from the Ministry of Science and Technology of P. R. China (Grant No. G1999012005).

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Authors and Affiliations

  1. College of Animal Science, Key Laboratory of Animal Husbandry Science and Technology of Xinjiang Production and Construction Corps, Tarim University, Alar, Xinjiang, 843300, People’s Republic of China

    Ailong Sha

  2. College of Life Science, LuDong University, Yantai, Shandong, 264025, People’s Republic of China

    Hushan Sun & Yiyan Wang

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  1. Ailong Sha
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  2. Hushan Sun
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Correspondence to Ailong Sha.

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Sha, A., Sun, H. & Wang, Y. Immunohistochemical Study of Leucine-Enkephalin and Delta Opioid Receptor in Mantles and Feet of the Octopus Octopus ocellatus Gray. Int J Pept Res Ther 18, 71–76 (2012). https://doi.org/10.1007/s10989-011-9280-x

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