Abstract
Marine Bacillus circulans DMS-2 (MTCC 8281) was found to produce lipopeptides, which reduced the surface tension of the medium from 69 to 28 mNm−1. The methanol extracts of the lipopeptides were resolved in RP-HPLC, which resulted in the presence of two major surface active fractions corresponding to the peaks E and F were eluted at the retention times of 16.8 and 18 min, respectively. Fourier transform infrared spectroscopy and matrix assisted laser desorption ionization time of flight (MALDI-ToF) mass spectral analysis showed that the produced lipopeptides belonged to surfactin family. In the MALDI-ToF spectrum, the major molecular mass of the HPLC purified isoforms were identified at m/z 1,044 and 1,058 Da, respectively. In addition, this marine strain also produced new variants of surfactins with molecular weights ranging from m/z 1,066 to 1,098 Da, which have not been reported earlier. Both surface-active fractions were found to have potent antimicrobial activity against the Gram-positive and Gram-negative bacterial strains.
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Acknowledgements
RS and CS acknowledge the Department of Biotechnology (DBT), Government of India for the project grant (BT/PR-6827/AAQ/03/263/2005) in marine biotechnology. SM acknowledges CSIR, New Delhi for the Senior Research Fellowship. Authors acknowledge Palashpriya Das for taking pains in strain isolation and for her all-round support throughout this work. CS acknowledges Ramapati Samanta, Technical Staff, Department of Biotechnology, IIT Kharagpur. The authors gratefully acknowledge Dr. Rukhsana Chowdhury for her great support in performing mass analysis at MALDI-ToF research facility of IICB, Kolkata.
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Sivapathasekaran, C., Mukherjee, S. & Sen, R. Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectral Analysis of Marine Lipopeptides with Potential Therapeutic Implications. Int J Pept Res Ther 16, 79–85 (2010). https://doi.org/10.1007/s10989-010-9206-z
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DOI: https://doi.org/10.1007/s10989-010-9206-z