Abstract
Calsequestrin (Casq) is a high capacity, low affinity Ca2+-binding protein, critical for Ca2+-buffering in cardiac and skeletal muscle sarcoplasmic reticulum. All vertebrates have multiple genes encoding for different Casq isoforms. Increasing interest has been focused on mammalian and human Casq genes since mutations of both cardiac (Casq2) and skeletal muscle (Casq1) isoforms cause different, and sometime severe, human pathologies. Danio rerio (zebrafish) is a powerful model for studying function and mutations of human proteins. In this work, expression, biochemical properties cellular and sub-cellular localization of D. rerio native Casq isoforms are investigated. By quantitative PCR, three mRNAs were detected in skeletal muscle and heart with different abundances. Three zebrafish Casqs: Casq1a, Casq1b and Casq2 were identified by mass spectrometry (Data are available via ProteomeXchange with identifier PXD002455). Skeletal and cardiac zebrafish calsequestrins share properties with mammalian Casq1 and Casq2. Skeletal Casqs were found primarily, but not exclusively, at the sarcomere Z-line level where terminal cisternae of sarcoplasmic reticulum are located.
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Abbreviations
- B2M:
-
Beta-2 microglobulin
- casq :
-
Zebrafish calsequestrin genes
- Casq:
-
Zebrafish calsequestrin proteins
- DTT:
-
DL-Dithiothreitol
- EF1a:
-
Elongation factor 1-alpha
- NDUFS3:
-
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3
- EGTA:
-
Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid
- HEPES:
-
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
- MOPS:
-
4-Morpholinepropanesulfonic acid
- PMSF:
-
Phenylmethylsulfonyl fluoride
- Ryr:
-
Ryanodine receptor
- SDS:
-
Sodium dodecyl sulfate
- SERCA1:
-
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
- SR:
-
Sarcoplasmic reticulum
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Acknowledgments
We thank Matthias Mann for his support in Mass Spectrometry data generation and analysis, Martina Milanetto, Korbinian Mayr, Igor Paron and Gabriele Sowa for their technical assistance. This work was supported by research funds from the University of Padova (ex 60 %).
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Furlan, S., Mosole, S., Murgia, M. et al. Calsequestrins in skeletal and cardiac muscle from adult Danio rerio . J Muscle Res Cell Motil 37, 27–39 (2016). https://doi.org/10.1007/s10974-015-9432-2
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DOI: https://doi.org/10.1007/s10974-015-9432-2