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Use of isothermal microcalorimetry to characterize the behavior of the microencapsulated biocontrol agent Meyerozyma guilliermondii

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Abstract

The effect of the microencapsulation by spray drying of the biocontrol agent Meyerozyma guilliermondii was evaluated by isothermal microcalorimetry (IMC) and traditional microbiological methods. The concentration of wall materials (soy protein isolate and sodium alginate) and inlet air temperature of the spray dryer were evaluated on the yeast viability, release rate and inhibition of Colletotrichum gloeosporioides. The parameters obtained by IMC such as maximum growth rate, lag phase time, total heat produced and the time to peak obtained from the heat flow curves were also analysed. The results showed an effect of the inlet air temperature and SPI concentration on the patterns of the heat flow curves vs. time, as well as in the kinetic parameters obtained from them. An inversely proportional correlation was found between the lag phase time and the parameters of release rate and percentage of mycelial growth inhibition. The total heat produced and the maximum growth rate was correlated with the percentage of inhibition. Likewise, the total heat produced was also correlated with the release rate. In this study, we found that higher activity of the yeast (measured in terms of heat produced and maximum growth rate by IMC) indicates a higher antifungal activity against C. gloeosporioides.

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Acknowledgements

This study was financially supported by CONACYT (Mexico) whom the authors thank for the scholarship granted to Rafael López-Cruz (305354) and to the support through the project No. 316948, and Tecnologico Nacional de Mexico through the project No. 7868.20-P. This research is part of the activities of the CYTED network N319RT0576 “Desarrollo sostenible en agroalimentacion y aprovechamiento de residuos industriales”.

Funding

This study was financially supported by CONACYT (Mexico) whom the authors thank for the scholarship granted to Rafael López-Cruz (305354) and through the Project 316948, and Tecnologico Nacional de Mexico through the Project No. 7868.20-P. This research is part of the activities of the CYTED network N319RT0576 “Desarrollo sostenible en agroalimentacion y aprovechamiento de residuos industriales”.

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Authors and Affiliations

  1. Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México/Instituto Tecnológico de Tepic, Av. Tecnológico 2595, Col. Lagos del Country, C.P. 63175, Tepic, Nayarit, Mexico

    Rafael López-Cruz, Juan Arturo Ragazzo-Sánchez & Montserrat Calderón-Santoyo

  2. Center for Biomechanics and Biocalorimetry, University of Basel, Gewerbestrasse 14-16, 4123, Allschwil, Switzerland

    Olivier Braissant

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  1. Rafael López-Cruz
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  2. Juan Arturo Ragazzo-Sánchez
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  3. Olivier Braissant
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  4. Montserrat Calderón-Santoyo
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by RL-C. The first draft of the manuscript was written by RL-C and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Montserrat Calderón-Santoyo.

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López-Cruz, R., Ragazzo-Sánchez, J.A., Braissant, O. et al. Use of isothermal microcalorimetry to characterize the behavior of the microencapsulated biocontrol agent Meyerozyma guilliermondii. J Therm Anal Calorim 147, 12569–12576 (2022). https://doi.org/10.1007/s10973-022-11456-8

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