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Microcalorimetric study of the effect of artesunate on the growth metabolism of mitochondria isolated from rat liver

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Abstract

In this study, the metabolic thermogenic curves of mitochondria isolated from rat liver tissue were drawn, and the effects of artesunate on metabolic activity of mitochondria were studied using TAM Air Isothermal Microcalorimeter. After isolation from the rat liver, mitochondria still have metabolic activity and can live for a week with appropriate nutrients. With the addition of artesunate (7.5 μM/mL), the metabolic activity of mitochondria was completely lost at this concentration. From the thermogenic curves, we obtained the thermokinetic equations under different conditions: T max = 2512.24 + 1761.53c, R = 0.9375; and Tlag (min) = 707.26 + 285.17c, R = 0.9846. The results showed that artesunate was a mitochondria-targeted drug having obvious effects on mitochondrial metabolism.

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Acknowledgements

The authors do gratefully acknowledge the financial support provided by the Natural Science Foundation of Guangxi, China (No. 0728227), the Funded Research Project of Guangxi Education Department (No. 201012MS171), and the Graduate Education Innovation Project of Guangxi (No. 201135556) for this study.

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Authors and Affiliations

  1. College of Pharmacy, Guilin Medical University, Guilin, Guangxi, 541004, People’s Republic of China

    Xuesong Shen, Meihua Jin, Chunxia Zhao, Xinqiang Tan, Hanfu Liu, Xuelian Qin & Zhuangpingi Qiu

  2. Chemistry and Molecule Scientific Institute, Wuhan University, Wuhan, 430072, People’s Republic of China

    Yi Liu

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  1. Xuesong Shen
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  2. Meihua Jin
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  3. Chunxia Zhao
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Correspondence to Xuesong Shen.

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Shen, X., Jin, M., Zhao, C. et al. Microcalorimetric study of the effect of artesunate on the growth metabolism of mitochondria isolated from rat liver. J Therm Anal Calorim 111, 1947–1952 (2013). https://doi.org/10.1007/s10973-011-2079-3

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  • DOI: https://doi.org/10.1007/s10973-011-2079-3

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