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Investigation of copper(II) complexation by glycylglycine using isothermal titration calorimetry

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Abstract

Isothermal titration calorimetry (ITC) and potentiometric titration methods have been used to study the process of proton transfer in the copper(II) ion-glycylglycine reaction. The stoichiometry, conditional stability constants, and thermodynamic parameters (ΔG, ΔH, and ΔS) for the complexation reaction were determined using the ITC method. The measurements were carried out at 298.15 K in solutions with a pH of 6 and the ionic strength maintained with 100 mM NaClO4. Carrying out the measurements in buffer solutions of equal pH but different enthalpies of ionization of its components (Mes, Pipes, Cacodylate) enabled determination of the enthalpy of complex formation, independent of the enthalpy of buffer ionization. The number of protons released by glycylglycine on account of complexation of the copper(II) ions was determined from calorimetric and potentiometric measurements.

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Acknowledgements

This research was supported by the Polish Ministry of Science and Higher Education under grant DS/8230-4-0088-10 and N N204 136238.

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Authors and Affiliations

  1. Faculty of Chemistry, University of Gdańsk, Sobieskiego 18, 80-952, Gdańsk, Poland

    D. Wyrzykowski, D. Zarzeczańska, D. Jacewicz & L. Chmurzyński

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  1. D. Wyrzykowski
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  2. D. Zarzeczańska
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  3. D. Jacewicz
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  4. L. Chmurzyński
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Correspondence to D. Wyrzykowski.

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Wyrzykowski, D., Zarzeczańska, D., Jacewicz, D. et al. Investigation of copper(II) complexation by glycylglycine using isothermal titration calorimetry. J Therm Anal Calorim 105, 1043–1047 (2011). https://doi.org/10.1007/s10973-011-1426-8

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  • DOI: https://doi.org/10.1007/s10973-011-1426-8

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