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Utilization of the IC-calorimeter for study of enzymatic reaction

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Abstract

In this article, the monitoring of an enzymatic reaction by means of a miniaturized batch type IC-calorimeter was performed. The aim of this work was focused on an investigation of enthalpy and rate of enzymatic reaction of trypsin with Nα-benzoyl-l-arginine-p-nitroanilide hydrochloride (BApNA). Both the parameters were determined for reactions in different buffers and for varying concentration of enzyme at 37 °C. The rate of reaction decreased with the increasing concentration of enzyme caused by trypsin autolysis.

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References

  1. Lerchner J, Wolf A, Hüttl R, Wolf G. Direct monitoring of biochemical processes using micro-structured heat power detectors. Chem Eng J. 2004;101:187–94.

    Article  CAS  Google Scholar 

  2. Lerchner J, Wolf A, Wolf G. Recent developments in integrated circuit calorimetry. J Therm Anal Calorim. 1999;57:241–51.

    Article  CAS  Google Scholar 

  3. Wadsö I. Characterization of microbial activity in soil by use of isothermal microcalorimetry. J Therm Anal Calorim. 2009;95:843–50.

    Article  Google Scholar 

  4. Antonelli ML, Lelo D, Tornelli RF. A microcalorimetric enzymatic method for trehalose determination in food. J Therm Anal Calorim. 2009;97:835–8.

    Article  CAS  Google Scholar 

  5. Baier V, Födisch R, Ihring A. Highly sensitive thermopile heat power sensor for micro-fluid calorimetry of biochemical processes. Sen Actuators A. 2005;123:354–5.

    Article  Google Scholar 

  6. Xensor Integration. Nano calorimeters for liquid (online). Last update 23-6-2009. [cit. 19-8-2009]. www.xensor.nl/pdffiles/sheets/nanoliq.pdf.

  7. Lerchner J, Wolf G, Auguet C, Torra V. Accuracy in integrated circuit (IC) calorimeters. Thermochim Acta. 2002;382:65–76.

    Article  CAS  Google Scholar 

  8. Wright MR. An introduction to chemical kinetics. Chichester: Wiley; 2005. p. 365–6.

  9. Bugg T. An introduction to enzyme and coenzyme chemistry. Oxford: Blackwell Science;1997. p. 16.

  10. Kodíček M, Karpenko V. Biofysikální chemie. Prague: Academia Praha; 2000.

  11. Podzemná V, Kourková L, Svoboda L. Microcalorimeter-essential aspects of the measurement. Sci Pap Univ Pardubice Ser A. 2009;15:137–8.

    Google Scholar 

  12. Gabel D, Kasche V. Autolysis of beta-trypsin. Influence of calcium ions and heat. Acta Chem Scand. 1973;27(6):1971–81.

    Article  CAS  Google Scholar 

  13. Guilbault GG. Analytical uses of immobilized biological compounds for detection, medical and industrial uses. New York: Marcel Dekker; 1984.

    Google Scholar 

  14. Cobb AK, Novotný M. High-sensitivity peptide mapping by capillary zone electrophoresis and microcolumn liquid chromatography, using immobilized trypsin for protein digestion. Anal Chem. 1989;67:2226–31.

    Article  Google Scholar 

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Acknowledgements

Authors thank to Dr. J. Lerchner and A. Wolf for their help and useful suggestions. The financial support from the Ministry of Education, Youth and Sports of Czech Republic under the project MSM0021627501 is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Inorganic Technology, Faculty of Chemical Technology, University of Pardubice, Nám. Čs. Legií 565, 532 10, Pardubice, Czech Republic

    V. Podzemná, L. Kourková & L. Svoboda

  2. Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 530 09, Pardubice, Czech Republic

    M. Slováková

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  1. V. Podzemná
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  2. M. Slováková
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  3. L. Kourková
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  4. L. Svoboda
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Correspondence to V. Podzemná.

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Podzemná, V., Slováková, M., Kourková, L. et al. Utilization of the IC-calorimeter for study of enzymatic reaction. J Therm Anal Calorim 101, 715–719 (2010). https://doi.org/10.1007/s10973-010-0951-1

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