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Application of microcalorimetry and principal component analysis

Antibacterial evaluation of Benzoinum and Styrax on Staphylococcus aureus growth

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Abstract

A useful microcalorimetric technique based on the bacterial heat production was applied to evaluate the antibacterial effects of Benzoinum and Styrax on the growth of Staphylococcus aureus (S. aureus). The thermogenic power-time curves of S. aureus growth in the presence of the two drugs were determined by a thermal activity monitor (TAM) air isothermal microcalorimeter, ampoule mode, at 310 K. Some quantitative metabolic parameters, such as growth rate constant k, the heat-flow power P, the appearance time for the heat power t, and the heat production Q were obtained from these curves. By analyzing these curves and some quantitative parameters using principal component analysis (PCA), the antibacterial effects of Benzoinum and Styrax on S. aureus growth were accurately evaluated from the change of the two main parameters, the heat-flow power for the second peaks P 2nd and total heat production Q t: the antibacterial effects of the two drugs at concentrations of 0–125 mg mL−1 were both enhanced with increasing the concentration, and Benzoinum with IC50 of 132.2 mg mL−1 had stronger antibacterial effect than Styrax with IC50 of 179.8 mg mL−1. This study provides some useful references for the application of Benzoinum and Styra as potential antibacterial agents. Microcalorimetry is a powerful analytical tool for the characterization of the microbial growth progress and the evaluation of the drugs’ efficiency.

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References

  1. China Pharmacopoeia Committee. Pharmacopoeia of the People’s Republic of China, 1st Div. 2005 ed. Chinese Chemical Industry Press, Beijing; 2005. p. 101 and 113–114.

  2. Coppen JJW. Benzoin: production, uses and international trade. Perfum Flavor. 1999;24:11–22.

    Google Scholar 

  3. Filippi JJ, Castel C, Fernandez X, Rouillard M, Gaysinski M, Lavoine-Hanneguelle S. An unusual acenaphthylene-type sesquiterpene hydrocarbon from Siam and Sumatra benzoin gum. Phytochem Lett. 2009;2:216–9.

    Article  CAS  Google Scholar 

  4. Fritsch PW. Phylogeny of Styrax based on morphological characters with implications for biogeography and infrageneric classification. Syst Bot. 1999;24:356–78.

    Article  Google Scholar 

  5. Moon HI, Lee J, Chung JH. The effect of erythrodiol-3-acetate on the expressions of matrix metalloproteinase-1 and type-1 procollagen caused by ultraviolet irradiated cultured primary old aged human skin fibroblasts. Phytomedicine. 2006;13:707–11.

    Article  CAS  Google Scholar 

  6. Min BS, Oh SR, Ahn KS, Kim JH, Lee J, Kim DY, Kim EH, Lee HK. Anti-complement activity of norlignans and terpenes from the stem bark of Styrax japonica. Planta Med. 2004;70:1210–5.

    Article  CAS  Google Scholar 

  7. Min BS, Na MK, Oh SR, Ahn KS, Jeong GS, Li G, Lee SK, Joung H, Lee HK. New furofuran and butyrolactone lignans with antioxidant activity from the stem bark of Styrax japonica. J Nat Prod. 2004;67:1980–4.

    Article  CAS  Google Scholar 

  8. Chowdhry BZ, Beezer AE, Greehow EJ. Calorimetry in the biological sciences. Talanta. 1983;30:209–12.

    Article  CAS  Google Scholar 

  9. Rodríguez de Rivera M, Socorro F. Flow microcalorimetry and thermokinetics of liquid mixtures. J Therm Anal Calorim. 2007;87:591–4.

    Article  Google Scholar 

  10. Liu W, Chaspoul F, Lefranc DB, Decome L, Gallice P. Microcalorimetry as a tool for Cr(VI) toxicity evaluation of human dermal fibroblasts. J Therm Anal Calorim. 2007;89:21–4.

    Article  CAS  Google Scholar 

  11. Khvedelidze M, Mdzinarashvili T, Partskhaladze T, Nafee N, Schaefer UF, Lehr CM, Schneider M. Calorimetric and spectrophotometric investigation of PLGA nanoparticles and their complex with DNA. J Therm Anal Calorim. 2010;99:337–48.

    Article  CAS  Google Scholar 

  12. Zhao YL, Yan D, Wang JB, Zhang P, Xiao XH. Anti-fungal effect of berberine on Candida albicans by microcalorimetry with correspondence analysis. J Therm Anal Calorim. doi 10.1007/s10973-009-0565-7.

  13. Boling EA, Blanchard GC, Russcal WJ. Microcalorimetric study the growth of bacteria. Nature. 1973;241:472–3.

    Article  CAS  Google Scholar 

  14. Kong WJ, Zhao YL, Shan LM, Xiao XH, Guo WY. Thermochemical studies on the quantity-antibacterial effect relationship of four organic acids from Radix Isatidis on Escherichia coli growth. Biol Pharm Bull. 2008;31:1301–5.

    Article  CAS  Google Scholar 

  15. Batovska D, Parushev ST, Slavova A, Bankova V, Tsvetkova I, Ninova M. Study on the substituents’ effects of a series of synthetic chalcones against the yeast Candida albicans. Eur J Med Chem. 2007;42:87–92.

    Article  CAS  Google Scholar 

  16. Liu Y, Tan AM, Xie CL, Wang CX. Thermokinetic study of bacterial arithmetic series growth. Acta Phys Chim Sin. 1996;12:451–5.

    Google Scholar 

  17. Leng P, Guo XL, Yang Y, Lou HX. Primary study on anti-fungal activities and reversal of fluconazole resistance of Plagiochin E. Chin Pharm J. 2007;42:349–52.

    CAS  Google Scholar 

  18. Kong WJ, Wang JB, Jin C, Zhao YL, Dai CM, Xiao XH, Li ZL. Effect of emodin on Candida albicans growth investigated by microcalorimetry combined with chemometric analysis. Appl Microbiol Biotechnol. 2009;83:1183–90.

    Article  CAS  Google Scholar 

  19. Kong WJ, Zhao YL, Xiao XH, Li ZL, Ren YS. Action of palmatine on Tetrahymena thermophila BF5 growth investigated by microcalorimetry. J Hazard Mater. 2009;168:609–13.

    Article  CAS  Google Scholar 

  20. Wadsö I. Characterization of microbial activity in soil by use of isothermal microcalorimetry. J Therm Anal Calorim. 2009;95:843–50.

    Article  Google Scholar 

  21. Kong WJ, Zhao YL, Shan LM, Xiao XH, Guo WY. Microcalorimetric studies of the action of four organic acids in Radix isatidis on the growth of microorganisms. Chin J Biotechnol. 2008;24:646–50.

    Article  CAS  Google Scholar 

  22. Massart DL, Vandeginste BGM, Deming SN, Michotte Y, Kaufman L. Chemometrics: a textbook. New York, NY: Elsevier; 1988.

    Google Scholar 

  23. Kong WJ, Zhao YL, Xiao XH, Li ZL, Jin C, Li HB. Investigation of the anti-fungal activity of coptisine on Candida albicans growth by microcalorimetry combined with principal component analysis. J Appl Microbiol. 2009;107:1072–80.

    Article  CAS  Google Scholar 

  24. Ivosev G, Burton L, Bonner R. Dimensionality reduction and visualization in principal component analysis. Anal Chem. 2008;80:4933–44.

    Article  CAS  Google Scholar 

  25. Alonso-Salces RM, Héberger K, Holland MV, Moreno-Rojas JM, Mariani C, Bellan G, Reniero F, Guillou C. Multivariate analysis of NMR fingerprint of the unsaponifiable fraction of virgin olive oils for authentication purposes. Food Chem. 2010;118:956–65.

    Article  CAS  Google Scholar 

  26. Kong WJ, Jin C, Xiao XH, Zhao YL, Li ZL, Zhang P, Liu W, Li XF. Comparative study of effects of two bile acid derivatives on Staphylococcus aureus by multiple analytical methods. J Hazard Mater. 2010;179:742–7.

    Article  CAS  Google Scholar 

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Acknowledgements

We are grateful to the support of National Basic Research Program of China (973 project) (2007CB512606). We thank the reviewers for their critical comments on the manuscript.

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Authors and Affiliations

  1. Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, People’s Republic of China

    Jian Wang, Danhong Cheng, Nan Zeng, Houlin Xia & Yong Fu

  2. China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing, 100039, People’s Republic of China

    Danhong Cheng, Dan Yan, Yanling Zhao & Xiaohe Xiao

Authors
  1. Jian Wang
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  2. Danhong Cheng
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  3. Nan Zeng
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  4. Houlin Xia
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  5. Yong Fu
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  6. Dan Yan
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  7. Yanling Zhao
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  8. Xiaohe Xiao
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Correspondence to Jian Wang.

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Wang, J., Cheng, D., Zeng, N. et al. Application of microcalorimetry and principal component analysis. J Therm Anal Calorim 102, 137–142 (2010). https://doi.org/10.1007/s10973-010-0929-z

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  • DOI: https://doi.org/10.1007/s10973-010-0929-z

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