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Physico-chemical changes taking place in gamma irradiated bovine globulins studied by thermal analysis

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Abstract

Structural transformations induced in gamma and alpha globulins under influence of gamma irradiation using doses of 2.5 and 24 kGy were studied by differential scanning calorimetry (DSC) and thermogravimetry (TG, DTG). Thermal decomposition of the globulins irradiated in water suspensions occurs at higher temperatures, in comparison to the reference non-irradiated samples. This was related to formation of covalent linkages in the irradiated proteins, apart to chemical changes induced in amino-acids. Essential modification of thermal decomposition was detected already after irradiation with a dose of 2.5 kGy performed for water suspensions. Irradiation of solid native proteins induces decrease in decomposition temperature and gives evidence of proteins degradation.

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Acknowledgements

The authors would like to thank to Dr. Wojciech Głuszewski from the Institute of Nuclear Chemistry and Technology for gamma irradiation.

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Authors and Affiliations

  1. Institute of Nuclear Chemistry and Technology, Dorodna 16 str., 03-195, Warszawa, Poland

    Krystyna Cieśla

  2. Department of Chemistry, University of Antwerp, Universiteitplein, 2610, Wilrijk, Belgium

    Etienne F. Vansant

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  1. Krystyna Cieśla
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  2. Etienne F. Vansant
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Correspondence to Krystyna Cieśla.

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Cieśla, K., Vansant, E.F. Physico-chemical changes taking place in gamma irradiated bovine globulins studied by thermal analysis. J Therm Anal Calorim 99, 315–324 (2010). https://doi.org/10.1007/s10973-009-0155-8

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  • DOI: https://doi.org/10.1007/s10973-009-0155-8

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