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Microcalorimetric investigation of the growth of the engineering BACILLUS THURINGIENSIS with different plasmid numbers and various promoters

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Abstract

A microcalorimetric technique based on the bacterial heat output was applied to evaluate the influence of plasmid copy number and various promoters on the green fluorescent protein expression in Bacillus thuringiensis. The thermogenic curves of the aerobic metabolism of B. thuringiensis strains were determined by using an LKB-2277 bioactivity monitor at 28°C. The analysis of the thermogenic curves indicated for the first time that the more plasmid copy number per cell the more protein synthetization. Promoter BtI-BtII had a stronger impact on the gene expression than promoter 3A investigated by the method of microcalorimetry.

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References

  1. M. L. H. Gruwel, C. Alves and J. N. Schrader, Am. J. Physiol., 268 (1995) H351.

    Google Scholar 

  2. O. Culic, M. L. H. Gruwel and J. Schrader, Am. J. Physiol., 273 (1997) C205.

    Google Scholar 

  3. R. J. Miles, A. E. Beezer and D. H. Lee, Microbiol., 45 (1986) 7.

    Google Scholar 

  4. Y. Liu, X. Li, S.S. Qu and Shen Ping, J. Biochem. Biophys. Methods, 45 (2000) 231.

    Article  Google Scholar 

  5. L. Ruan, Y. Liu, Z. Gao and P. Shen, J. Therm. Anal. Cal., 70 (2002) 521.

    Article  Google Scholar 

  6. X. Y. Lin, Huazhong Agriculture University, Wuhan, China: M.S. Thesis (2003) 33.

  7. J. Suurkuusk and I. WadsØ. Chem. Scr., 20 (1992) 155.

    Google Scholar 

  8. Y. Yang, Y. Liu, J. Zhu and P. Shen, J. Therm. Anal. Cal., 75 (2004) 293.

    Article  Google Scholar 

  9. Y. P. Huang, Y. Liu, P. Shen and S. S. Qu, J. Therm. Anal. Cal., 74 (2003) 163.

    Article  Google Scholar 

  10. X. Y. Lin, Y. Liu, M. Sun and Z. T. Gao, Acta Chim. Sinica, 59 (2001) 769.

    Google Scholar 

  11. M. Itaya, M. Syed, S. Haheduzzaman, K. Matsui, A. Omori and T. Tsuji, Biosci. Biotechnol. Biochem., 65 (2001) 579.

    Article  Google Scholar 

  12. H. W. Park, B. X. Ge and L. S. Baner, Appl. Environ. Microbiol., 64 (1998) 3932.

    Google Scholar 

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Authors and Affiliations

  1. State Key Laboratory of Agricultural Microbiology of China, Huazhong Agricultural University Wuhan, 430070, P. R. China

    L. Xiaoyan, S. Ming, L. Lin & Y. Ziniu

  2. Department of Chemistry, College of Chemistry and Molecular Sciences, Wuhan University Wuhan, 430072, P. R. China

    L. Yi, L. Peng, Z. Juncheng & Q. Songsheng

Authors
  1. L. Xiaoyan
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  2. L. Yi
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  3. S. Ming
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  4. L. Peng
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  5. Z. Juncheng
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  6. L. Lin
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  7. Q. Songsheng
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  8. Y. Ziniu
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Correspondence to Y. Ziniu.

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Xiaoyan, L., Yi, L., Ming, S. et al. Microcalorimetric investigation of the growth of the engineering BACILLUS THURINGIENSIS with different plasmid numbers and various promoters. J Therm Anal Calorim 79, 29–32 (2005). https://doi.org/10.1007/s10973-004-0557-6

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  • DOI: https://doi.org/10.1007/s10973-004-0557-6

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