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Chitosan-co-acrylic acid microgel fabricated with green synthesized silver nanoparticles for the sensing of hydrogen peroxide

  • Original Paper: Sol-gel and hybrid materials for catalytic, photoelectrochemical and sensor applications
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Abstract

A novel, fast, stable, and cost-effective colorimetric hydrogen peroxide (H2O2) sensor was prepared by the incorporation of silver nanoparticles (AgNPs) in chitosan-co-acrylic acid (CS-co-AAc) microgel. AgNPs were synthesized by green method by using Euphorbia Maculate (EM) leaf extract. Chitosan-co-acrylic acid/silver nanaoparticle (CS-co-AAc/AgNPs) hybrid microgel can be characterized by various analytical techniques. CS-co-AAc/AgNPs hybrid microgel exhibited UV-visible absorption band 436 nm. X-ray diffraction spectrum reveals that CS-co-AAc/AgNPs hybrid microgel are amorphous in nature and the crystalline nature of synthesized AgNPs. The hybrid microgels are highly selective towards H2O2 and the absorption band intensity is proportional to the concentration of H2O2 in solution. Over the concentration range of 0–50 nM, the intensity of the absorption peak decreases linearly,and the corresponding correlation coefficient is R2 -0.9063. The limit of detection is determined to be 3.2 nM. Antibacterial activities of the hybrid microgel were also observed against aquatic bacteria such as Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) isolated from river water samples taken from Kerala. Practical applicability of the hybrid microgel for the sensing of H2O2 in real milk samples was also investigated. AgNPs themselves can show similar behaviors, but they are destabilized because it undergo rapidly agglomerate. This hybrid microgel system offers numerous advantages, such as sample processing and extreme stability. This technique is ideal for assessing diverse biological and environmental materials since it is easy to use, inexpensive, and adaptable to complex matrices.

Graphical Abstract

The integration of green synthesized AgNPs in CS-co-AAc microgels resulted in a unique, rapid, stable, and cost-effective colorimetric sensor for H2O2. EM leaf extract was used to synthesize AgNPs. Analytical tests of the CS-co-AAc/AgNPs hybrid microgel show that AgNPs are successfully incorporated into the microgel. Hybrid microgel exhibits a UV–vis. absorption peak at 436 nm and selective for H2O2, with a level of detection of 3.2 nM. Its shows excellent antibacterial activities are more effective against S. aureus bacteria than against E. coli bacteria. The practical application of hybrid microgel for H2O2 sensing in actual milk was also explored. The results show that the sensors have a higher selectivity toward H2O2 at low concentrations and a higher sensitivity than previously reported H2O2 sensors.

Highlights

  • Fabrication of green synthesized silver nanoparticle incorporated to CS-co-AAc microgel.

  • CS-co-AAc /AgNPs hybrid microgel was characterized by various analytical techniques.

  • Hybrid microgel able to quantify H2O2 in milk samples.

  • Antibacterial activity of hybrid microgel was explored towards waterborne bacteria.

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Acknowledgements

Third author is grateful to Department of Science & Technology (DST), Government of India for instrumentation facilities provided by DST-PURSE Phase II.

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Authors and Affiliations

  1. Department of Chemistry, St. Joseph’s College, Moolamattom, Idukki, 685591, Kerala, India

    Neenu George & Ebey P. Koshy

  2. Department of Chemistry, Newman College, Thodupuzha, Idukki, 685585, Kerala, India

    Jithin Joy

  3. School of Chemical Sciences, Mahatma Gandhi University, Kottayam, 686560, India

    Beena Mathew

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George, N., Joy, J., Mathew, B. et al. Chitosan-co-acrylic acid microgel fabricated with green synthesized silver nanoparticles for the sensing of hydrogen peroxide. J Sol-Gel Sci Technol 107, 685–696 (2023). https://doi.org/10.1007/s10971-023-06104-y

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