Skip to main content
SpringerLink
Log in

Immobilization of papain on mesoporous silica: pH effect

  • Original Article
  • Published:
Journal of Sol-Gel Science and Technology Aims and scope Submit manuscript

Abstract

The enzyme papain was adsorbed on mesoporous silica at 4°C and pH values between 3 and 11. From the adsorption kinetic data, the rate constant for the process was evaluated. The concentration of the enzyme in solution was calculated by monitoring the absorbance at 280 nm, and the quantity of papain bound to the solid was evaluated from the initial and residual soluble enzyme concentrations. The best results were observed for papain adsorption at pH 5.0; under these conditions, the desorption of the enzyme was <5%.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Y.F. Li, F.Y. Jia, J.R. Li, G. Liu, and Y.Z. Li, Biotechnol. Appl. Biochem. 33(Pt1), 29 (2001).

    CAS  Google Scholar 

  2. J. Wang, D. Bhattachargya, and L.G. Bachas, Biomacromolecules 2(3), 700 (2001).

    CAS  Google Scholar 

  3. R. Ganapathy, S. Mandache, M. Sarmadi, and F. Denes, J. Biomater. Sci. Polym. 12(9), 1027 (2001).

    CAS  Google Scholar 

  4. K.K. Unger, Porous Silica, its Properties and Use as Support in Column Liquid Chromatography (Elsevier, Amsterdan, 1979).

    Google Scholar 

  5. R.K. Iler, The Chemistry of Silica (A Wiley-Interscience Publishers, New York, 1979).

    Google Scholar 

  6. A.P. Legrand (ed.), The surface properties of Silicas (J. Wiley & Sons Ltd., New York, 1998).

    Google Scholar 

  7. M. Huckel, H.J. Wirth, M.T. Hearn, J. Biochem. Biophys. Methods 31, 165 (1996).

    Article  CAS  Google Scholar 

  8. S. Liu, R.P. Hanzlik, Biochem. Biophys. Acta 1158, 264 (1993).

    CAS  Google Scholar 

  9. H.H. Weetall, Biochim. Biophys. Acta 185, 464 (1969).

    CAS  Google Scholar 

  10. E. Papirer, Adsorption on Silica Surfaces (Marcel Dekker, Inc., 2000).

  11. G.V. Lyubinskii, Ukr. Biokhim. Zhurn. 56, 390 (1984).

    CAS  Google Scholar 

  12. R.D. Shannon, J. Appl. Phys. 73, 348 (1993).

    Article  CAS  Google Scholar 

  13. R.W. Pickersgill, P.W. Goodenough, I.G. Sumner, and M.E. Collins, Biochem. J. 254, 235 (1988).

    CAS  Google Scholar 

  14. K. Brocklehurst, J. Carlsson, M.P.J. Kierstan, and E.M. Crook, Biochem. J. 133, 573 (1973).

    CAS  Google Scholar 

  15. S.S. Husain and G. Lowe, Biochem. J. 114, 279 (1969).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, Universidad Autonoma Metropolitana-Iztapalapa, P.O. Box 55-534, Mexico, D. F., 09340, Mexico

    S. Solís, J. Paniagua, J. C. Martínez & M. Asomoza

Authors
  1. S. Solís
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Paniagua
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. C. Martínez
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Asomoza
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Solís, S., Paniagua, J., Martínez, J.C. et al. Immobilization of papain on mesoporous silica: pH effect. J Sol-Gel Sci Technol 37, 125–127 (2006). https://doi.org/10.1007/s10971-006-6431-1

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10971-006-6431-1

Keywords

Search

Navigation