Abstract
This paper describes efforts of the structural genomics project in the nuclear magnetic resonance (NMR) laboratory at the University of Science and Technology of China. This structural genomics project is biological-functional driven. Targets are mainly selected from two systems: proteins related with regulation of gene expression in humans and other eukaryotes, and proteins existing in the cell junction in humans. The majority of proteins selected from these two systems are related with human health and diseases, and some are potential drug targets. Twenty-five protein structures from Homo sapiens and other eukaryotes have been determined during last 5 years in this laboratory. Nuclear magnetic resonance (NMR) spectroscopy is highly suited to investigate molecular interactions at a close physiological condition and is particularly suited for the study of low-affinity, transient complexes. It can provide information on protein surface interaction, their complex structure, and their dynamic properties during protein recognition. Several examples are given in this paper.
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Acknowledgements
This work was supported by the Chinese National Fundamental Research Project (Grant 2002CB713806, 2006CB806507 and 2006CB910201), the Chinese National Natural Science Foundation (Grant 30121001, 30570361 and 30670426), the Key Project of the National High Technology Research and Development Program of China (Grant 2002BA711A13), and the Pilot Project of the Knowledge Innovation Program of the Chinese Academy of Science (Grant KSCX1-SW-17). The authors would like to thank Jiahai Zhang, Chunlei Pu, and Yajun Tang for technical support and thank graduate students who contributed to the work.
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Shi, Y., Wu, J. Structural basis of protein–protein interaction studied by NMR. J Struct Funct Genomics 8, 67–72 (2007). https://doi.org/10.1007/s10969-007-9021-8
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DOI: https://doi.org/10.1007/s10969-007-9021-8