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The simple method of lipolysis/lipogenesis balance assessment based on 14C isotope: advances and importance of pH control

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Abstract

Our study is devoted to the development of an optimal protocol for lipolysis and lipogenesis assessment using 14C-labeled glucose. We confirmed the utility of the developed method for the analysis of lipogenesis and lipolysis rates in adipocytes using insulin and isoproterenol. The present study describes rapid, cheap and simple techniques for the assessment of lipids metabolism and storage in adipocytes. Upgraded methods of 14C-labeled TAG extraction and saponification allow to investigate mechanisms of lipolysis, lipogenesis and TAG-cycling.

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Abbreviations

ATGL:

Adipose tissue triglyceride lipase

ANOVA:

Analysis of variance

CE:

Cholesterol esters

Ces1:

Carboxylesterase type 1

DMEM HG:

Dulbecco modified Eagle’s medium high glucose

DAG:

Diacylglycerols

FBS:

Fetal bovine serum

FA:

Fatty acids

FFA:

Free fatty acids

HSL:

Hormone-sensitive lipase

MAG:

Monoacylglycerols

MGL:

Monoacylglycerol lipase

NMR:

Nuclear magnetic resonance

RIPA:

Radioimmunoprecipitation assay

TAG:

Triacylglycerols

TLC:

Thin layer chromatography

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Acknowledgements

This work was supported by Russian Science Foundation Grant #22-75-10085.

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Author notes

  1. S. Michurina and M. Agareva have equally contributed to this work.

Authors and Affiliations

  1. National Medical Research Centre of Cardiology Named After Academician E.I.Chazov, Moscow, Russia, 121552

    S. Michurina, M. Agareva, E. Ratner, M. Menshikov, I. Stafeev & Ye. Parfyonova

  2. Lomonosov Moscow State University, Moscow, Russia, 119991

    S. Michurina & Ye. Parfyonova

  3. Institute of Fine Chemical Technologies Named After M.V. Lomonosov, Moscow, Russia, 119571

    M. Agareva

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  1. S. Michurina
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Contributions

S.M. and M.A. collected and analyzed the data, wrote and reviewed the manuscript. E.R. and M.M. analyzed the data and reviewed the manuscript. I.S. wrote, reviewed the manuscript and conceptualized the study. Ye.P. reviewed the manuscript and conceptualized the study.

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Correspondence to I. Stafeev.

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Michurina, S., Agareva, M., Ratner, E. et al. The simple method of lipolysis/lipogenesis balance assessment based on 14C isotope: advances and importance of pH control. J Radioanal Nucl Chem 333, 125–134 (2024). https://doi.org/10.1007/s10967-023-09198-4

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  • DOI: https://doi.org/10.1007/s10967-023-09198-4

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