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γ-radiation-induced damage on normal hepatocytes and its protection by ethyl cinnamate

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Abstract

This study aimed to investigate the efficacy of ethyl cinnamate (EC) (75 μg/ml) in ameliorating the effects of γ-radiation (2, 5, and 8 Gy) on normal liver BRL-3A cells at 1 and 24 h post-irradiation. γ-radiation-induced persistent damage to normal hepatocytes is partly mediated by reactive oxygen species (ROS)-regulated early apoptosis and prolonged inhibition of survival factors. EC treatment increased superoxide dismutase (SOD) activity, decreased ROS yield and lipid peroxidation (LPO), activated the PI3-AKT pathway and p-Bcl-2, and reduced cleaved caspase -3,  - 9, and Bax. This study highlights for the first time the potential of ethyl cinnamate as an efficient radioprotector.

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Acknowledgements

The author AD thankfully acknowledges the University Grant Commission (UGC), Government of India, for funding the project (Sanction no. F.15-6 Dec 2014/2015 NET). The author SM would like to thank the Indian Council of Medical Research (ICMR) for providing a research grant (No. 3/1/3/JRF-2016/HRD). The authors thank the UGC-DAE Consortium for Scientific Research, Kolkata Centre, India, for providing all the facilities for conducting this research.

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  1. Sharmi Mukherjee and Anindita Dutta: Joint first authors.

Authors and Affiliations

  1. Division of Radiation and Stress Biology, UGC-DAE Consortium for Scientific Research, Kolkata Centre, Block-LB, Plot-8, Sector-III, Bidhan Nagar, Kolkata, West Bengal, 700106, India

    Sharmi Mukherjee, Anindita Dutta & Anindita Chakraborty

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  1. Sharmi Mukherjee
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Contributions

Conceptualization: SM, AD, AC; Methodology: SM, AD; Formal analysis and investigation: SM, AD; Writing—original draft preparation: SM, AD; Writing—review and editing: SM, AD, AC; Funding acquisition: SM, AD; Supervision: AC All authors have read and approved the final manuscript.

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Correspondence to Anindita Chakraborty.

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Mukherjee, S., Dutta, A. & Chakraborty, A. γ-radiation-induced damage on normal hepatocytes and its protection by ethyl cinnamate. J Radioanal Nucl Chem 333, 1453–1465 (2024). https://doi.org/10.1007/s10967-023-09067-0

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