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Design of docetaxel-loaded polymeric nanoparticles: characterization, radiolabeling with 99mTc and in vitro evaluation

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Abstract

This research aims to develop a polymeric drug delivery system for docetaxel (DTX) and to investigate its radiotracer potential by in vitro. DTX loaded Poly(lactic acid)/Poly(ethylene glycol) diblock copolymer (PLA–PEG) was synthesized and radiolabeled with technetium-99m tricarbonyl core. PLA–PEG–DTX nanoparticles had a particle size and zeta potential 120 ± 7.3 nm and − 24.66 ± 1.5 mV respectively, which was further supported by SEM image. The radiolabeling efficiency of 99mTc(CO)3-PLA–PEG–DTX was found to be 90 ± 3.51%,. In vitro study was carried out on cancer cell lines (MCF7, MDA-MB-231) to examine the biological behavior of docetaxel loaded PLA–PEG nanoparticles.

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Acknowledgements

The authors thank Ph.D. Eser Uçar for scientific english editing of the manuscript. MCF7 and MDA-MB-231 cells were obtained from Ege University, Faculty of Science, Department of Biochemistry.

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Authors and Affiliations

  1. Department of Material Science and Engineering, Graduate Faculty of Natural and Applied Science, Ege University, Bornova, 35100, İzmir, Turkey

    Oğuz Çetin & Serap Teksöz

  2. Department of Nuclear Applications, Institute of Nuclear Sciences Ege University, Bornova, 35100, İzmir, Turkey

    Çiğdem İçhedef & Burcu Aydın

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  1. Oğuz Çetin
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  2. Çiğdem İçhedef
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Çetin, O., İçhedef, Ç., Aydın, B. et al. Design of docetaxel-loaded polymeric nanoparticles: characterization, radiolabeling with 99mTc and in vitro evaluation. J Radioanal Nucl Chem 326, 1639–1652 (2020). https://doi.org/10.1007/s10967-020-07454-5

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  • DOI: https://doi.org/10.1007/s10967-020-07454-5

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