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Low-dose gamma irradiation induces water activity, leaf K+/Na+, glycine betaine, antioxidant enzyme activity and reduces lipid peroxidation and protease activity to enhance salt tolerance in pigeonpea [Cajanus cajan (L.) Millsp]

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Abstract

Soil salinity is a major constraint that limits legume productivity. The present study evaluates the physiological and biochemical basis of radiation affect on salt tolerance response of pigeonpea. Seed gamma irradiation, in general, at 1.92 (control), 5.86 (80 mM) and 8.02 dSm−1 (100 mM) soil electrical conductivity (NaCl stress), enhanced seedling establishment, plant growth, carbon metabolism and gas exchange characteristics such as net photosynthesis, stomatal conductance and transpiration rate. Further, an improvement in salt tolerance response of gamma irradiated (<10 Gy) pigeonpea was related to high seed water activity and leaf K+/Na+, glycine betaine, membrane stability index and enzymic antioxidant activity.

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Abbreviations

EC:

Electrical conductivity

Aw:

Water activity

MSI:

Membrane stability index

SOD:

Superoxide dismutase

CAT:

Catalase

POX:

Peroxidase

Pn:

Photosynthetic rate

Gs:

Stomatal conductance

E:

Transpiration rate

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Acknowledgments

The authors are thankful to the Head, Environmental Science and Head, Plant Physiology, Indian Agricultural Research Institute, New Delhi for providing instrument facility. The scholarship provided to the first author by the Indian Agricultural Research Institute for doctoral degree is gratefully acknowledged.

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Authors and Affiliations

  1. Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, 110 012, India

    Pankaj Kumar & Vasundhara Sharma

  2. Division of Genetics and Plant Breeding, Indian Agricultural Research Institute, New Delhi, 110 012, India

    R. S. Raje

  3. Nuclear Research Laboratory, Indian Agricultural Research Institute, New Delhi, 110 012, India

    Bhupinder Singh

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  1. Pankaj Kumar
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Correspondence to Bhupinder Singh.

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Kumar, P., Sharma, V., Raje, R.S. et al. Low-dose gamma irradiation induces water activity, leaf K+/Na+, glycine betaine, antioxidant enzyme activity and reduces lipid peroxidation and protease activity to enhance salt tolerance in pigeonpea [Cajanus cajan (L.) Millsp]. J Radioanal Nucl Chem 308, 965–980 (2016). https://doi.org/10.1007/s10967-015-4596-1

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