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Radiocomplexation and evaluation of the 99mTc-Gemifloxacin in artificially Escherichia coli infected mice

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Abstract

Gemifloxacin as a broad spectrum quinolone antibacterial agent was radiocomplexed with high activity of 99mTc and was evaluated as an infection imaging agent in artificially Escherichia coli (E. Coli) infected mice. 99mTc-Gemifloxacin with high specific activity (0.148 GBq/μmol) and labeling yield (98.60 ± 0.70 %) was obtained. Our main achievement was high accumulation in the E. Coli infected right thigh muscle in mice (T/NT = 1.89 at 4 h post injection) which may diagnostically be beneficial to distinguish sites of E. Coli infection.

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Acknowledgments

The authors would like to thank Mr. Mazidi and Mr Talebi for their excellent technical assistance in experiments and biodistribution studies.

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Authors and Affiliations

  1. Radiation Application Research School, Nuclear Science and Technology Research Institute (NSTRI), North Kargar Ave, P.O. Box: 14395-836, Tehran, Iran

    M. Erfani & M. Shafiei

  2. Department of Basic Science, Faculty of Veterinary, Science and Research Branch, Islamic Azad University, P.O. Box: 14515-775, Tehran, Iran

    M. Rekabgardan & P. Mortazavi

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  1. M. Erfani
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  2. M. Rekabgardan
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Correspondence to M. Erfani.

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Erfani, M., Rekabgardan, M., Mortazavi, P. et al. Radiocomplexation and evaluation of the 99mTc-Gemifloxacin in artificially Escherichia coli infected mice. J Radioanal Nucl Chem 308, 825–833 (2016). https://doi.org/10.1007/s10967-015-4515-5

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