Skip to main content
SpringerLink
Log in

Metabolic stability and biological evaluation of 99mTc-HYNIC-Tyr3-Octreotide as somatostatin receptor positive tumor imaging agent

  • Published:
Journal of Radioanalytical and Nuclear Chemistry Aims and scope Submit manuscript

Abstract

In this study, metabolic stability and biological evaluation of 99mTc labelled 6-hydrazinopyridine-3-carboxylic-acid (HYNIC)-Tyr3-Octreotide was reported as a candidate for imaging somatostatin-receptor (SSTR)-positive tumors. The metabolic stability analysis did not show any evidence of breaking of the labeled compound and formation of free 99mTc. In biodistribution and scintigraphy imaging study, radiopeptide have showed high and receptor-specific uptake in the SSTR2 positive organs, tumor with rapid renal excretion from non-target tissues, illustrating the excellent properties of this agent for SSTR tumor imaging.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Liu S, Edwards DS, Barrett JA (1997) Bioconj Chem 8(5):621–636

    Article  CAS  Google Scholar 

  2. Reubi JC, Schar JC, Waser B, Wenger S, Heppeler A, Schmitt JS, Macke HR (2000) Eur J Nucl Med Mol Imaging 27(3):273–282

    Article  CAS  Google Scholar 

  3. Bakker WH, Krenning EP, Breeman WA, Kooij PPM, Reubi JC, Koper JW, Dejong M, Lameris JS, Visser TJ, Lamberts SW (1991) J Nucl Med 32(6):1184–1189

    CAS  Google Scholar 

  4. Krenning EP, Bakker WH, Kooij PPM, Breeman WAP, Oei HY, Dejong M, Reubi JC, Visser TJ, Bruns C, Kwekkeboom DJ, Reijs AEM, Vanhagen PM, Koper JW, Lamberts SWJ (1992) J Nucl Med 33(5):652–658

    CAS  Google Scholar 

  5. Breeman WAP, de Jong M, Kwekkeboom DJ, Valkema R, Bakker WH, Kooij PPM, Visser TJ, Krenning EP (2001) Eur J Nucl Med Mol Imaging 28(9):1421–1429

    Article  CAS  Google Scholar 

  6. Liu S, Edwards DS (1999) Chem Rev 99(9):2235–2268

    Article  CAS  Google Scholar 

  7. Maina T, Nock B, Nikolopoulou A, Sotiriou P, Loudos G, Maintas D, Cordopatis P, Chiotellis E (2002) Eur J Nucl Med Mol Imaging 29(6):742–753

    Article  CAS  Google Scholar 

  8. Behera A, De K, Chandra S, Chattopadhyay S, Misra M (2011) J Radioanal Nucl Chem 290(1):123–129

    Article  CAS  Google Scholar 

  9. Gabriel M, Decristoforo C, Maina T, Nock B, vonGuggenberg E, Cordopatis P, Moncayo R (2004) Cancer Biother Radiopharm 19(1):73–79

    Article  CAS  Google Scholar 

  10. Behera A, Banerjee I, De K, Munda RN, Misra M, Chattopadhayay S, Samanta A, Sarkar B, Ganguly S (2013) Amino Acids 44:933–946

    Article  CAS  Google Scholar 

  11. Ernst O, Zor T (2010) J Vis Exp. 2010(38):1918

    Google Scholar 

  12. Berges JA, Fisher AE, Harrison PJ (1993) Mar Biol 115(2):187–193

    Article  CAS  Google Scholar 

  13. Decristoforo C, Melendez-Alafort L, Sosabowski JK, Mather SJ (2000) 99mTc-HYNIC- yr (3)-octreotide for imaging somatostatin-receptor-positive tumors: preclinical evaluation and comparison with 111In-octreotide. J Nucl Med 41(6):1114–1119

    CAS  Google Scholar 

  14. Maina T, Nock BA, Cordopatis P, Bernard BF, Breeman WAP, van Gameren A, van den Berg R, Reubi JC, Krenning EP, de Jong M (2006) 99mTc Demotate 2 in the detection of SST (2) -positive tumors: a preclinical comparison with 111In DOTA-Tate. Eur J Nucl Med Mol Imaging 33(7):831–840

    Article  Google Scholar 

  15. Gandomkar M, Najafi R, Shafiei M, Mazidi M, Ebrahimi SES (2007) Preclinical evaluation of 99mTc/EDDA/tricine/HYNIC0, 1-Nal(3), Thr(8)-octreotide as a new analogue in the detection of somatostatin-receptor-positive tumors. Nucl Med and Biol 34(6):651–657

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors gratefully acknowledge Prof. Siddhartha Roy, Ex-Director, CSIR-Indian Institute of Chemical Biology (CSIR-IICB), Kolkata, India and Dr. N. Sivaprasad, Ex-Sr. General Manager, Board of Radiation and Isotope Technology (BRIT), Navi Mumbai, India for their support. And special thanks to Mr. Indranil Banerjee, CSIR-Indian Institute of Chemical Biology (CSIR-IICB), Kolkata, India for kind support, during animal handling. Authors sincerely acknowledge Dr. Santanu Ganguly (Head of the Department of Nuclear Medicine), Bharat Ratna Sarkar and Samarendu Sinha of Regional Radiation Medicine Centre, Thakurpukur Cancer Research Institute, Kolkata, for their kind help in taking gamma camera images of rat. We also thank the Board of Research in Nuclear Sciences, Department of Atomic Energy (BRNS-DAE) and Council of scientific and industrial research (CSIR) for financial support.

Author information

Authors and Affiliations

  1. Infectious Disease and Immunology Division, Department of Nuclear Medicine, CSIR-Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Kolkata, 700032, India

    Ashok Behera, Kakali De & Mridula Misra

  2. Radiopharmaceuticals Laboratory, Regional Centre, Board of Radiation and Isotope Technology, Variable Energy Cyclotron Centre, 1/AF, Bidhan Nagar, Kolkata, 700064, India

    Sankha Chattopadhayay

Authors
  1. Ashok Behera
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kakali De
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sankha Chattopadhayay
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mridula Misra
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Ashok Behera or Mridula Misra.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Behera, A., De, K., Chattopadhayay, S. et al. Metabolic stability and biological evaluation of 99mTc-HYNIC-Tyr3-Octreotide as somatostatin receptor positive tumor imaging agent. J Radioanal Nucl Chem 304, 595–602 (2015). https://doi.org/10.1007/s10967-014-3867-6

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10967-014-3867-6

Keywords

Search

Navigation