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Proton induced X-ray emission (PIXE) technique for determining multi-element composition of transformed hairy root cultures of Boerhaavia diffusa L.: an important medicinal herb

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Abstract

Therapeutically important inorganic elements in Agrobacterium rhizogenes-mediated genetically transformed hairy root cultures (HRCs) of a pharmaceutically significant herb Boerhaavia diffusa were quantified using proton induced X-ray emission technique. This was compared with that of roots from the naturally grown donor plant. Two macro-elements (Ca & K) and eight different trace elements namely V, Cr, Mn, Fe, Co, Cu Zn, and Ni were detected and their content was determined. In HRCs of a transformed rhizoclone, calcium and potassium had values which were significantly higher than that of in vivo roots. The concentrations of several trace elements, which are known to have a positive implication in human healthcare, were found to be either comparable (Fe) to that in the natural root samples or higher (Mn, Zn, Cr, Cu, Co) in the transformed rhizoclone. The genetically transformed HRCs can thus serve as a fast-proliferating renewable resource of medicinally useful minerals targeting specific diseases.

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Authors and Affiliations

  1. SFS Applied Microbiology, Post-Graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar, 751 004, Odisha, India

    Lopamudra Sahu & Pradeep K. Chand

  2. Ion Beam Laboratory, Institute of Physics, Bhubaneswar, 751 005, Odisha, India

    Dinesh K. Ray

  3. Department of Chemistry, Utkal University, Vani Vihar, Bhubaneswar, 751 004, Odisha, India

    Dinesh K. Ray

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  1. Lopamudra Sahu
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  2. Dinesh K. Ray
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  3. Pradeep K. Chand
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Correspondence to Pradeep K. Chand.

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Sahu, L., Ray, D.K. & Chand, P.K. Proton induced X-ray emission (PIXE) technique for determining multi-element composition of transformed hairy root cultures of Boerhaavia diffusa L.: an important medicinal herb. J Radioanal Nucl Chem 300, 345–354 (2014). https://doi.org/10.1007/s10967-014-3022-4

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  • DOI: https://doi.org/10.1007/s10967-014-3022-4

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