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Evaluation of labelling conditions, quality control and biodistribution study of 99mTc-5-aminolevulinic acid (5-ALA): a potential liver imaging agent

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Abstract

Labelling of 5-aminolevulinic acid (5-ALA) with 99mTc was achieved by using SnCl2·2H2O as reducing agent. Radiochemical purity and labelling efficiency was determined by instant thin layer chromatography/paper chromatography. Efficiency of labelling was dependent on many parameters such as amount of ligand, reducing agent, pH, and time of incubation. 99mTc labelled 5-ALA remained stable for 24 h in human serum. Tissue biodistribution of 99mTc-5-ALA was evaluated in Sprague–Dawley rats. Biodistribution study (% ID/g) in rats revealed that 99mTc-5-ALA was accumulated significantly in liver, spleen, stomach and intestine after half hour, 4 and 24 h. Significant activity was noted in bladder and urine at 4 h. High liver uptake of 99mTc-5-ALA makes it a promising liver imaging agent.

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Authors and Affiliations

  1. Pakistan Institute of Engineering and Applied Sciences, Islamabad, Pakistan

    Kalim Ullah Khan & Mohammad Rafi

  2. Isotope Production Division, Pakistan Institute of Nuclear Science and Technology, P.O. Nilore, Islamabad, 45650, Pakistan

    Samina Roohi, Rizwana Zahoor, Zafar Iqbal & Mushtaq Ahmad

Authors
  1. Kalim Ullah Khan
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  2. Samina Roohi
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  3. Mohammad Rafi
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  4. Rizwana Zahoor
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  5. Zafar Iqbal
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  6. Mushtaq Ahmad
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Correspondence to Mushtaq Ahmad.

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Khan, K.U., Roohi, S., Rafi, M. et al. Evaluation of labelling conditions, quality control and biodistribution study of 99mTc-5-aminolevulinic acid (5-ALA): a potential liver imaging agent. J Radioanal Nucl Chem 300, 225–228 (2014). https://doi.org/10.1007/s10967-014-3014-4

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  • DOI: https://doi.org/10.1007/s10967-014-3014-4

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