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PACAP-27 radioimmunoassay: Description and application of a novel method

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Abstract

Pituitary adenylate cyclase activating polypeptide (PACAP) is a widely distributed neuropeptide that has two molecular forms with 38 and 27 amino acid residues. The aim of the present study was to develop a new, highly specific PACAP-27 assay to investigate the quantitative distribution of PACAP-27 in the central nervous system of various vertebrate species applying the same technical and experimental conditions. Our results show that the antiserum used turned to be PACAP-27 specific. The average ID50 value was 51.5±3.6 fmol/ml and the detection limit was 2 fmol/ml. PACAP-27 immunoreactivity was present in the examined brain areas, with highest concentration in the rat diencephalon and telencephalon. Swine and pigeon brain also contained significant amount of PACAP-27. Our results confirm the previously described data showing that PACAP-38 is the dominant form of PACAP in vertebrates, since PACAP-38 levels exceeded those of PACAP-27 in all examined brain areas. Furthermore, our study describes for the first time, the comparative quantitative distribution of PACAP-27 and-38 in the swine and pigeon brain.

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Authors and Affiliations

  1. Department of Pharmacology and Pharmacotherapy, Neuropharmacology Research Group of the Hungarian Academy of Sciences, Pécs, Hungary

    J. Németh, B. Jakab & B. Horváth

  2. Department of Anatomy, Neurohumoral Regulations Research Group of the Hungarian Academy of Sciences, Pécs, Hungary

    R. Józsa, T. Hollósy, A. Tamás, A. Lubics, I. Lengvári, P. Kiss & D. Reglődi

  3. Department of Pediatrics, University of Pécs, Szigeti út 12, H-7624, Pécs, Hungary

    Zs. Oberritter

  4. Department of Pharmacology and Pharmacotherapy, University of Debrecen, Nagyerdei krt. 98, H-4019, Debrecen, Hungary

    Z. Szilvássy

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  1. J. Németh
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Németh, J., Jakab, B., Józsa, R. et al. PACAP-27 radioimmunoassay: Description and application of a novel method. J Radioanal Nucl Chem 273, 327–332 (2007). https://doi.org/10.1007/s10967-007-6862-3

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  • DOI: https://doi.org/10.1007/s10967-007-6862-3

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