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A novel pH-and temperature sensitive polymer based on MoS2 modified poly (N-Isopropyl Acrylamide)/ allyl acetoacetate for doxorubicin delivery: synthesis, characterization, in-vitro release and cytotoxicity studies

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Abstract

In this paper, we aimed to develop the nanocarrier based on poly (N-isopropyl acrylamide)—allyl acetoacetate grafted MoS2 nanosheets. The obtained polymer modified with methoxy poly (ethylene glycol) and folic acid to enable enhanced adsorption of doxorubicin. The synthesized polymer was characterized using Fourier transform infrared, X-ray diffraction, field emission scanning electron microscope, and thermogravimetric analysis. The effect of main experimental parameters on the doxorubicin adsorption were investigated and the maximum adsorption capacity was obtained at pH = 8, contact time = 15 min, and temperature = 50 °C. The results indicated that the doxorubicin release was considerably accelerated at a simulated cancer fluids (pH = 5.6) in contrast to simulated human blood fluids (pH = 7.4). Also, the cytotoxicity of the obtained nanocarrier was evaluated via MTT assay against KB cancer cell lines. The doxorubicin release and subsequent induction of apoptosis enhanced after near infrared irradiation, indicating that this nanocarrier can be employed as a dual responsive drug delivery system, with controlled drug release through near infrared irradiation and pH. The adsorption data followed the Langmuir isotherm model with a maximum adsorption capacity of 16.83 mg g−1. Kinetic studies revealed that the adsorption process was fitted with the pseudo-second-order model.

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Abbreviations

DOX:

Doxorubicin

DDSs:

Drug delivery systems

PTT:

Photothermal therapy

NIR:

Near infrared

MoS2 :

Molybdenum disulfide

TMDCs:

Transition- metal di-chalcogenides

pNIPAm:

Poly (N-isopropylacrylamide)

PEG:

Poly (ethylene glycol)

MPEG:

Methoxy poly (ethylene glycol)

FA:

Folic acid

FR:

Folic acid receptors

ABIN:

2,2, Azobisisobutyronitrile

DMF:

Dimethylformamide

AAA:

Allyl acetoacetate

DI:

Deionized

FTIR:

Fourier transform infrared

XRD:

X-ray diffraction

FE-SEM:

Field emission scanning electron microscope

EDX:

Energy-dispersive X-ray analysis

TGA:

Thermogravimetric analysis: TGA;

ICP-MS:

Inductively coupled plasma-mass spectrometry: ICP-MS

PBS:

Phosphate-buffered saline

DMEM:

Dulbecco’s modified Eagle’s medium

DMSO:

Fetal bovine serum: FBS; Dimethyl sulfoxide

OD:

Optical density

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Acknowledgements

The authors would like to acknowledge Islamic Azad University (North Tehran Branch) for financial support of this project.

Funding

The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.

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Authors and Affiliations

  1. Department of Chemistry, North Tehran Branch, Islamic Azad University, Tehran, Iran

    Elham Reza Soltani & Kambiz Tahvildari

  2. Department of Chemistry, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran

    Elham Moniri

  3. Department of Chemistry, Central Tehran Branch, Islamic Azad University, Tehran, Iran

    Homayon Ahmad Panahi

Authors
  1. Elham Reza Soltani
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  2. Kambiz Tahvildari
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Contributions

Elham Reza Soltani: Methodology, Conceptualization, Investigation, Formal analysis, Validation, Writing-original draft. Kambiz Tahvildari: Supervision, Project administration, Formal analysis, Validation, Writing-original draft. Elham Moniri: Supervision, Project administration, Formal analysis, Validation, Writing-original draft. Homayon Ahmad Panahi: Project administration, Formal analysis, Writing-review and editing, Investigation.

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Correspondence to Kambiz Tahvildari or Elham Moniri.

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Reza Soltani, E., Tahvildari, K., Moniri, E. et al. A novel pH-and temperature sensitive polymer based on MoS2 modified poly (N-Isopropyl Acrylamide)/ allyl acetoacetate for doxorubicin delivery: synthesis, characterization, in-vitro release and cytotoxicity studies. J Polym Res 29, 446 (2022). https://doi.org/10.1007/s10965-022-03286-x

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