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Synthesis of bovine serum albumin-gelatin composite adhesive hydrogels by physical crosslinking

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Abstract

In this work, a series of bovine serum albumin-gelatin (BSA-Gel) adhesive hydrogels were synthesized via non-covalent interactions. We demonstrated possible tuning the properties, microstructure, thermal stability of hydrogels by varying BSA concentration and Gel molecular weight. Two Gel molecular weights were used, which was confirmed using gel permeation chromatography (GPC) (M1, Mw ≈ 105 kDa, M2, Mw ≈ 2.9 kDa). The lap shear test showed that adhesive strength of 45% BSA-20% Gel (M1) and 45% BSA-20% Gel (M2) on gelatin coated glass slides was 384.4 kPa and 357.9 kPa respectively, which was higher than pure 20% Gel (M1), 20% Gel (M2) of 306.1 kPa and 321 kPa respectively. SEM results demonstrated that BSA-Gel (M1) hydrogels had compact structure, while BSA-Gel (M2) hydrogels had porous structure. The 45% BSA-20% Gel (M1) hydrogel was stable more than 10 h at 37 °C, which was promising for applications in many biomedical areas such as tissue adhesive, wound dressing and drug delivery.

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Acknowledgements

This research was supported by special fund of Guangdong academy of sciences (No. 2020GDASYL-20200103042, No. 2019GDASYL-0103020, 2020GDASYL-20200102005 and 2021GDASYL-20210102004), National Natural Science Foundation of China (No. 21907037), Guangdong Research and Development Plan in Key Areas (No. 2020B1111560001).

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Authors and Affiliations

  1. Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Shiliugang Road 10, Guangzhou, 510316, China

    Cuiping Guo, Zhiwen Zeng, Shan Yu, Jun Huang, Zhijie Geng, Dating Pei & Daohuan Lu

  2. National Engineering Research Center for Healthcare Devices, Guangzhou Road 1307, Guangzhou, 510500, China

    Cuiping Guo, Zhiwen Zeng, Shan Yu, Jun Huang, Zhijie Geng, Dating Pei & Daohuan Lu

  3. School of Pharmacy, Jinan University, Huangpu Avenue West 601, Guangzhou, 510632, China

    Cuiping Guo

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The manuscript entitled “Synthesis of bovine serum albumin-gelatin composite adhesive hydrogels by physical crosslinking” by Cuiping Guo, Zhiwen Zeng, Shan Yu, Jun Huang, Zhijie Geng, Dating Pei and Daohuan Lu is not having any conflict of interest.

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Guo, C., Zeng, Z., Yu, S. et al. Synthesis of bovine serum albumin-gelatin composite adhesive hydrogels by physical crosslinking. J Polym Res 29, 276 (2022). https://doi.org/10.1007/s10965-022-03130-2

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