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β-Cyclodextrin Encapsulates Biochanin A and Influences its Binding to Bovine Serum Albumin: Alteration of the Binding Strength

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Abstract

We report the host–guest association of Biochanin A with β-cyclodextrin and the modulation of its binding by cyclodextrin to bovine serum albumin. The binding constant of the complex is large. The strength of the binding of Biochanin A to bovine serum albumin decreases when β-cyclodextrin complexes with it. The binding constants of the Biochanin–serum albumin binding in the absence and the presence of cyclodextrin are (2.23 ± 0.08) × 106 and (1.37 ± 0.05) × 106 mol−1·dm3, respectively. The FRET studies reveal the decrease in the distance between the donor and the acceptor in the presence of cyclodextrin.

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Authors and Affiliations

  1. Department of Chemistry, Karunya University, Coimbatore, Tamil Nadu, 641 114, India

    N. Sudha, Y. Sameena & Israel V. M. V. Enoch

  2. Department of Nanosciences & Technology, Karunya University, Coimbatore, Tamil Nadu, 641 114, India

    Israel V. M. V. Enoch

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  1. N. Sudha
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Correspondence to Israel V. M. V. Enoch.

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Sudha, N., Sameena, Y. & Enoch, I.V.M.V. β-Cyclodextrin Encapsulates Biochanin A and Influences its Binding to Bovine Serum Albumin: Alteration of the Binding Strength. J Solution Chem 45, 431–444 (2016). https://doi.org/10.1007/s10953-016-0446-1

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