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Spectroscopic Investigation of the Interaction of Pyridinium Surfactant with Bovine Serum Albumin

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Abstract

The interaction of 1-dodecyl carbamoyl methylene pyridinium chloride (DAPC) with bovine serum albumin (BSA) was investigated by UV–Vis absorption, CD and fluorescence spectroscopies. The results of fluorescence titration reveal that DAPC strongly quenched the intrinsic fluorescence of BSA and caused a blue shift of the emission wavelength through a static quenching mechanism. The reduction of the binding constant (K A) and number of binding sites (n) between DAPC and BSA was studied with increasing temperature. The binding process is exothermic and entropy driven. The distance r between the donor of BSA and the acceptor of DAPC decreases with increasing concentration of DAPC. Furthermore, CD spectra and synchronous fluorescence spectra shows that DAPC induced conformational changes of BSA.

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Acknowledgments

This work was supported by the Science Research Project of the Ministry of Education of Heilongjiang Province of China (2012TD012, 12511Z030), the National Natural Science Foundation of Heilongjiang Province (B201114, B201313) and the Science Research Project of Key Laboratory of Fine Chemicals of College of Heilongjiang Province of China (JX201210).

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  1. Key Laboratory of Fine Chemicals of College of Heilongjiang Province, College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar, 161006, People’s Republic of China

    Zijia Liu, Xiangfeng Guo, Zhuo Feng & Lihua Jia

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  1. Zijia Liu
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  2. Xiangfeng Guo
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  3. Zhuo Feng
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  4. Lihua Jia
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Correspondence to Xiangfeng Guo or Lihua Jia.

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Liu, Z., Guo, X., Feng, Z. et al. Spectroscopic Investigation of the Interaction of Pyridinium Surfactant with Bovine Serum Albumin. J Solution Chem 44, 293–306 (2015). https://doi.org/10.1007/s10953-015-0304-6

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  • DOI: https://doi.org/10.1007/s10953-015-0304-6

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