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Study on the Interaction of Pentachlorophenol with Urease in Aqueous Solution by Multiple Spectroscopic Techniques

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Abstract

The interactions between pentachlorophenol (PCP) and jack bean urease were studied using UV/vis absorption, CD, fluorescence, synchronous fluorescence, and three-dimensional fluorescence spectroscopic techniques. The fluorescence data showed that the fluorescence quenching of urease by PCP the results of the formation of a PCP–urease complex involving a hydrophobic interaction. The distance r between the donor (urease) and acceptor (PCP) was obtained from the fluorescence resonance energy transfer. The effect of PCP on the conformation of urease was analyzed using UV/vis absorption, synchronous fluorescence and three-dimensional fluorescence spectroscopic techniques. The result showed that PCP can enter into the hydrophobic pocket at the interface of urease and that the micro environments around the tyrosine and tryptophan residues were changed.

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Authors and Affiliations

  1. Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng City, Jiangsu Province, 224002, People’s Republic of China

    Yan-Qing Wang

  2. Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, Jiangsu Province, 224002, People’s Republic of China

    Yan-Qing Wang, Gen-Cheng Zhang & Hong-Mei Zhang

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  1. Yan-Qing Wang
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  2. Gen-Cheng Zhang
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  3. Hong-Mei Zhang
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Correspondence to Yan-Qing Wang.

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Wang, YQ., Zhang, GC. & Zhang, HM. Study on the Interaction of Pentachlorophenol with Urease in Aqueous Solution by Multiple Spectroscopic Techniques. J Solution Chem 40, 458–469 (2011). https://doi.org/10.1007/s10953-011-9664-8

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  • DOI: https://doi.org/10.1007/s10953-011-9664-8

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