Skip to main content
SpringerLink
Log in

Fluorescence Spectral Study on the Interaction Between Copper(II) Complex with (E)-3-(2,3-Dihydrobenzo[b][1,4] Dioxin-6-Yl) Acrylic Acid and Urease

  • Published:
Journal of Structural Chemistry Aims and scope Submit manuscript

Abstract

We have previously reported the synthesis, structure and urease inhibitory activity of a complex [Cu2(L)4DMSO2]·2DMSO (HL = (E)-3-(2,3-dihydrobenzo[b][1,4]dioxin-6-yl)acrylic acid), and we found the complex to show a strong inhibitory activity against jack bean urease [1]. In this study, the interaction of the complex and urease is studied by fluorescence spectroscopy. We find that the fluorescence quenching mechanism of the complex with urease is static quenching. The quenching rate constant (Kq) is 1.6·1012 l/mol·s–1, the quenching constant (Ksv) is 1.6×104 l/mol, the association binding constant (K) is 3.55·104 l/mol, and the binding site number (n) is 1.22.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. X. F. Chen and C. F. Wang. J. Struct. Chem., 2017, 58(4), 841–847.

    Article  Google Scholar 

  2. H. L. Mobley and R. P. Hausinger. Microbiol. Rev., 1989, 53(1), 85–108.

    CAS  PubMed  PubMed Central  Google Scholar 

  3. H. L. Mobley, M. D. Island, and R. P. Hausinger. Microbiol. Rev., 1995, 59(30), 451–480.

    CAS  PubMed  PubMed Central  Google Scholar 

  4. L. E. Zonia and N. E. Stebbins. Adv. Plant Physiol., 1995, 107(4), 1097–1103.

    Article  CAS  Google Scholar 

  5. C. M. Collins and S. E. D'Orazio. Mol. Microbiol., 1993, 9(5), 907–913.

    Article  CAS  PubMed  Google Scholar 

  6. C. Montecucco and R. Rappuoli. Nat. Rev. Mol. Cell Biol., 2001, 2(6), 457–466.

    Article  CAS  PubMed  Google Scholar 

  7. Z. Wang, O. V. Cleemput, P. Demeyer, and L. Baert. Biol. Fertil. Soils, 1991, 11(1), 43–47.

    Article  CAS  Google Scholar 

  8. IARC Monograph on the Evaluation of the Carcinogenic Risks to Humans, 1994, 61(1).

  9. B. Krajewska. J. Mol. Catal. B: Enzym., 2009, 59(1-3), 22–40.

    Article  CAS  Google Scholar 

  10. B. J. Marshall. Gastroenterology Clinics of North America, 1993, 22(1), 183–98.

    CAS  PubMed  Google Scholar 

  11. A. R. Timerbaev, C. G. Hartinger, S. S. Aleksenko, and B. K. Keppler. Chem. Rev., 2006, 106(6), 2224–2248.

    Article  CAS  PubMed  Google Scholar 

  12. M. J. McKeage. Drug Saf., 1995, 13(4), 228–244.

    Article  CAS  PubMed  Google Scholar 

  13. H. M. Zhang, G. C. Zhang, and Y. Q. Wang. Biol. Trace Elem. Res., 2011, 141(1-3), 53–64.

    Article  CAS  PubMed  Google Scholar 

  14. J. R. Lakowicz. Principles of Fluorescence Spectroscopy, 3nd ed. New York: Springer, 2006.

    Book  Google Scholar 

  15. J. R. Lakowicz and G. Weber. Biochemistry, 1973, 12, 4161–4170.

    Article  CAS  Google Scholar 

  16. P. Mandal and T. Ganguly. J. Phys. Chem. B, 2009, 113, 14904–14913.

    Article  CAS  PubMed  Google Scholar 

  17. S. Wu, W. Yuan, H. Wang, Q. Zhang, M. Liu, and K. Yu. J. Inorg. Biochem., 2008, 102, 2026–2034.

    Article  CAS  PubMed  Google Scholar 

  18. J. R. Lakowicz. Principles of fluorescence spectroscopy, 2nd edn. New York: Plenum Press, 1999.

    Book  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Life Sciences, Shandong University of Technology, Zibo, P. R. China

    T.-R. Wang, J.-L. Ren, M.-J. Zhu, R.-M. Xie & G.-H. Sheng

  2. School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, P. R. China

    Q.-C. Zhou

Authors
  1. T.-R. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Q.-C. Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J.-L. Ren
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M.-J. Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R.-M. Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. G.-H. Sheng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to G.-H. Sheng.

Additional information

Original Russian Text © 2018 T.-R. Wang, Q.-C. Zhou, J.-L. Ren, M.-J. Zhu, R.-M. Xie, G.-H. Sheng.

The text was submitted by the authors in English. Zhurnal Strukturnoi Khimii, Vol. 59, No. 7, pp. 1732–1735, September-October, 2018.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, TR., Zhou, QC., Ren, JL. et al. Fluorescence Spectral Study on the Interaction Between Copper(II) Complex with (E)-3-(2,3-Dihydrobenzo[b][1,4] Dioxin-6-Yl) Acrylic Acid and Urease. J Struct Chem 59, 1674–1677 (2018). https://doi.org/10.1134/S0022476618070211

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0022476618070211

Keywords

Search

Navigation