Abstract
A thermodynamic study on the interaction of bovine carbonic anhydrase II, CAII, with cobalt(II) and iron(III) ions was made using isothermal titration calorimetry (ITC) at 300.15 K and 310.15 K in Tris buffer solutions at pH=7.5. The enthalpies of interaction of Co2++ CAII and Fe3++ CAII are reported and analyzed in terms of the extended solvation theory. The results indicate that there are three identical and non-cooperative binding sites for Co2+ and Fe3+ ions. Binding of these ions with CAII occurs exothermically with dissociation equilibrium constants of 87.15 and 91.00 μmol⋅L−1 at 300.15 K, for Co2+ and Fe3+, respectively.
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Open Access This is an open access article distributed under the terms of the Creative Commons Attribution Noncommercial License (https://creativecommons.org/licenses/by-nc/2.0), which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
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Rezaei Behbehani, G., Divsalar, A., Saboury, A.A. et al. A Thermodynamic Study on the Binding of Cobalt(II) and Iron(III) Ions with Bovine Carbonic Anhydrase II at Different Temperatures. J Solution Chem 39, 1142–1152 (2010). https://doi.org/10.1007/s10953-010-9572-3
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DOI: https://doi.org/10.1007/s10953-010-9572-3