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Effect of Ionic Strength and Temperature on the Protonation of Oxidized Glutathione

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Abstract

The protonation constants for oxidized glutathione, H i−1L(4−i+1)−, K H i =[H i L(4−i)−]/[H i−1L(4−i+1)−][H+] i=1,2,…,6 have been measured at 5, 25 and 45 °C as a function of the ionic strength (0.1 to 5.4 mol⋅[kg(H2O)]−1) in NaCl solutions. The effect of ionic strength on the measured protonation constants has been used to determine the thermodynamic values (K H0 i ) and the enthalpy (ΔH i ) for the dissociation reaction using the SIT model and Pitzer equations. The SIT (ε) and Pitzer parameters (β (0), β (1) and C) for the dissociation products (L4−, HL3−, H2L2−, H3L−, H4L, H5L+, H6L2+) have been determined as a function of temperature. These results can be used to examine the effect of ionic strength and temperature on glutathione in aqueous solutions with NaCl as the major component (body fluids, seawater and brines).

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Author notes

  1. Pasquale Crea

    Present address: Dipartimento di Chimica Inorganica, Chimica Analtica e Chimica Fisica, Universita di Messina, Salita Sperone 31, 98166, Messina (Vill. S. Agata), Italy

Authors and Affiliations

  1. Chemistry Department, Florida Institute of Technology, 150 West University Boulevard, Melbourne, FL, 32901, USA

    Pasquale Crea, Mutsa Kambarami & Virender K. Sharma

  2. Dipartimento di Chimica Inorganica, Chimica Analtica e Chimica Fisica, Universita di Messina, Salita Sperone 31, 98166, Messina (Vill. S. Agata), Italy

    Concetta De Stefano

  3. Rostenstiel School of Marine and Atmospheric Sciences, Marine and Atmospheric Chemistry, 4600, Rickenbacker Causeway, Miami, FL, 33149, USA

    Frank J. Millero

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  1. Pasquale Crea
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Correspondence to Virender K. Sharma.

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Crea, P., De Stefano, C., Kambarami, M. et al. Effect of Ionic Strength and Temperature on the Protonation of Oxidized Glutathione. J Solution Chem 37, 1245–1259 (2008). https://doi.org/10.1007/s10953-008-9310-2

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  • DOI: https://doi.org/10.1007/s10953-008-9310-2

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