Abstract
Zn-BDC (BDC = 1,4-benzenedicarboxylate acid) or more commonly known as metal organic framework (MOF-5) was synthesized via chemical stirring method and bioconjugated with anti-bovine serum albumin (anti-BSA) through pendent group. The pendent group of organic linker on surface of MOF-5 has been activated via EDC (1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide) based simple organic reaction and then bioconjugation of anti-BSA was achieved. Bioconjugation of anti-BSA on to MOF-5 has been used to study the interaction with BSA based on their fluorescence properties. Fluorescent microscopy revealed successful interaction of anti-BSA with BSA onto MOF-5 surface. In addition, BSA conjugated MOF-5 showed no loss of enantioselectivity and structure stability. Photoluminescence quenching of the developed probe was demonstrated to observe the presence of BSA.
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Acknowledgments
The reported work has been funded through CSIR, India Grant (OMEGA/PSC0202/2.2.5) from CSIR, India. One of the authors (Pawan Kumar) also acknowledges CSIR for his Senior Research Fellowship. Authors thank Mr. Parveen Kumar for his help in conducting the experimental works. We are also grateful to the Director, CSIR-CSIO, Chandigarh for providing facilities.
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Kumar, P., Deep, A., Paul, A.K. et al. Bioconjugation of MOF-5 for molecular sensing. J Porous Mater 21, 99–104 (2014). https://doi.org/10.1007/s10934-013-9752-9
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DOI: https://doi.org/10.1007/s10934-013-9752-9