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A Novel Glucose-6-Phosphate Isomerase Exists in Chicken Breast Meat: A Selenium-Containing Enzyme that Should Be Re-recognized Through New Eyes

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Abstract

Glucose-6-phosphate isomerase (GPI) is a highly conserved glycolytic enzyme in nature, and less information was available for GPI from hens. In this study a newly discovered selenocysteine (Sec)-containing GPI in common chicken breast meat was first isolated, purified and identified. Data about LC–MS/MS, FTIR and Se species analyses show that the molecular weight of the enzyme is 62,091 Da and only one Sec is inserted at the 403rd position in the highly conserved primary domain SIS_PGI with sugar conversion function. The enzyme shows excellent activity against hydroxyl radicals as vitamin C (Vc) in vitro. It is deduced that the Sec-containing GPI in the chicken meat may depend on Sec in its molecular structure to resist reactive oxygen species (ROS) stress produced by the accompanying biochemical reactions in cells, to protect its stability and maintain its efficient function that catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate in the critical glycolytic pathway.

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Acknowledgements

The paper was supported by Shaanxi Province Key R&D Program (2021SF-387) and Yulin Science and Technology Plan Project (2019-132).

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Authors and Affiliations

  1. College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Xin Hu, Chenxi Li, Yuancheng Li, Yi Jin, Lulu Wei, Xinlei Wang, Yanlong Xu & Zhongqiu Hu

  2. Laboratory of Quality & Safety Risk Assessment for Agro-Products (Yangling), Ministry of Agriculture and Rural Affairs of the People’s Republic of China, Yangling, 712100, Shaanxi, China

    Xin Hu, Chenxi Li, Yuancheng Li, Yi Jin, Lulu Wei, Xinlei Wang, Yanlong Xu & Zhongqiu Hu

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Xin Hu and Chenxi Li: Conceptualization and Writing-Original Draft; Yuancheng Li and Yi Jin: Validation; Lulu Wei and Xinlei Wang: Formal analysis; Yanlong Xu: Resources; Zhongqiu Hu: Funding and Supervision.

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Hu, X., Li, C., Li, Y. et al. A Novel Glucose-6-Phosphate Isomerase Exists in Chicken Breast Meat: A Selenium-Containing Enzyme that Should Be Re-recognized Through New Eyes. Protein J 42, 355–364 (2023). https://doi.org/10.1007/s10930-023-10105-9

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