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RETRACTED ARTICLE: Molecular Cloning, Sequence Characterization, and Tissue Expression Analysis of Hi-Line Brown Chicken Akirin2

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This article was retracted on 10 February 2012

Abstract

Akirins are novel important nuclear proteins able to modulate transcriptional activities in a gene-specific manner. Akirin2 is an important gene related to immune responses, it is necessary to isolate the akirin2 gene from chicken because it may be associated with vaccine and enhancement of immune response. In this study, a Hi-Line Brown chicken homolog of the vertebrate akirin2 gene was cloned, sequenced, and characterized. The akirin2 full-length coding sequence (CDS) consisted of 576nt and encoded 191 amino acids with a molecular weight of 21.58 kD. The COOH-terminal alpha-helix region was well conserved between chicken and other animals. RT-PCR analysis showed that the akirin2 transcripts were constitutively expressed in 16 tissues tested. Several microRNA target sites were predicted in the CDS of chicken akirin2 gene. We presume that Akirin2 protein may be used as a new-type immunopotentiator in poultry immune system in the future.

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Abbreviations

AA:

Aminos acid

CDS:

Coding sequence

NF-κB:

Nuclear factor-kappa B

Imd:

Immune deficiency

SC:

Satellite cell

IL:

Interleukin

SNP:

Single-nucleotide polymorphism

RT-PCR:

Reverse transcription-polymerase chain reaction

Mw:

Molecular weight

pI:

Isoelectric point

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Acknowledgments

This study was supported by Foundation of Heilongjiang Educational Committee (no. 12511140) and Young Scholars of Harbin Normal University (no. 10KXQ-06).

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Authors and Affiliations

  1. College of Life Science and Technology, Harbin Normal University, No. 1 South of Shida RD Limin Development Zone, Harbin, 150025, Heilongjiang, People’s Republic of China

    Chaolai Man, Xiang Li & Jongeun Lee

Authors
  1. Chaolai Man
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  2. Xiang Li
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  3. Jongeun Lee
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Corresponding author

Correspondence to Chaolai Man.

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This article has been retracted.

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Man, C., Li, X. & Lee, J. RETRACTED ARTICLE: Molecular Cloning, Sequence Characterization, and Tissue Expression Analysis of Hi-Line Brown Chicken Akirin2 . Protein J 30, 471–479 (2011). https://doi.org/10.1007/s10930-011-9352-y

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  • DOI: https://doi.org/10.1007/s10930-011-9352-y

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