Although TauA shares few common characteristics with other known periplasmic binding protein, TauA is a putative periplasmic binding protein, part of tauABCD gene cluster involved in sulfonate transport in sulphate starvation condition. This protein was expressed in E. coli BL 21 and purified before to assess its binding functionalities. Measurement of K d value (mean 11.3 nM) by binding/dialysis studies revealed high affinity and specificity with taurine and also indicated that TauA possessed a unique binding site for its ligand. Comparisons with other periplasmic binding proteins suggests TauA plays a major role in ABC transport system and could be ideal candidate to serve as taurine catcher in biological fluids.
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Abbreviations
- Cys:
-
cysteine
- E coli :
-
Eschericia coli
- ATP:
-
adenosine tri phospate
- HEPES:
-
4–(2-hydroxyethyl)-piperazine-1-ethanesulfonic acid
- MOPS:
-
3-(N-morpholino)-propanesulfonic acid
- IPTG:
-
isopropyl-β-thiogalactopyranoside
- IMAC:
-
immobilized metal ion chromatography
- TCA:
-
trichloroacetic acid
- HPLC:
-
high pressure liquid chromatography
- MWCO:
-
molecular weight cutt-off
- OD:
-
optical density
- Ssi:
-
sulfate starvation induced protein
- RT-PCR:
-
reverse transcriptase polymerase chain reaction
- ORF:
-
open reading frame
- LIV:
-
leucine-isoleucine-valine binding protein
- HBP:
-
histidine binding protein
- η:
-
the fractional saturation of protein molecule that are saturated with ligand
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Javaux, C., Joris, B. & De Witte, P. Functional Characteristics of TauA Binding Protein from TauABC Escherichia coli System. Protein J 26, 231–238 (2007). https://doi.org/10.1007/s10930-006-9064-x
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DOI: https://doi.org/10.1007/s10930-006-9064-x