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Development of a Novel Scaffold Based on Basil Seed Gum/Chitosan Hydrogel Containing Quercetin-Loaded Zein Microshphere for Bone Tissue Engineering

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Abstract

The chitosan-based hydrogel has high potential in tissue engineering; however, its performance is relatively poor in practice. Here, an easy method is introduced to produce high strength composite chitosan hydrogel. Chitosan (CS) and Basil seed gum (BSG) are converted into a 3-dimensional hydrogel with the assistance of Gallic acid (GA) as a cross-linking agent. The incorporation of GA into CS-BSG hydrogel forms cross-linking bonds (hydrogen bonds) between GA and polymer chains. The experimental results show that the GA cross-linked hydrogel has a dense microstructure, good mechanical properties, high porosity, thermal stability, and a high swelling ratio. With the high antioxidant activity and good biocompatibility, these properties make the GA-crosslinked hydrogel as a promising material for tissue engineering. Furthermore, adding quercetin loaded-Zein microsphere in the optimal hydrogel (the hydrogel containing the highest concentration of GA) proved to have a synergistic effect on the mentioned characteristics of the hydrogel. Cell culture tests confirmed that this scaffold is nontoxic and biocompatible for cell proliferation. The prepared hydrogels hold great potential as a scaffold for tissue engineering applications based on the results.

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Acknowledgements

The authors are thankful to the Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran (68391) for supported present study.

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Authors and Affiliations

  1. Department of Clinical Laboratory Science, College of Pharmacy, University of Al-Mustansiriyah, Baghdad, Iraq

    Mastafa H. Al-Musawi

  2. Department Pharmacology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

    Mohsen Rashidi

  3. The Health of Plant and Livestock Products Research Center, Mazandaran University of Medical Sciences, Sari, Iran

    Mohsen Rashidi

  4. Department of Chemistry, College of Science, University of Misan, Amarah, 62001, Iraq

    Salim Albukhaty

  5. College of Medicine, University of Warith Al-Anbiyaa, Karbala, Iraq

    Salim Albukhaty

  6. Research Center for Advanced Materials, Faculty of Materials Engineering, Sahand University of Technology, Tabriz, 5133511996, Iran

    Elham Mahmoudi

  7. Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Vahid Mohammadzadeh & Marjan Ghorbani

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Contributions

MHA-M: Writing—original draft, Methodology, Investigation. MR: Data Curation, review and editing, Formal analysis. VM: Data curation, Formal analysis, Methodology. SA: Review and editing, Formal analysis. EM: Review and editing, Formal analysis, Methodology. MG: Project administration, Conceptualization, Data curation, Supervision, Funding acquisition, review and editing, Formal analysis.

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Correspondence to Marjan Ghorbani.

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Al-Musawi, M.H., Rashidi, M., Mohammadzadeh, V. et al. Development of a Novel Scaffold Based on Basil Seed Gum/Chitosan Hydrogel Containing Quercetin-Loaded Zein Microshphere for Bone Tissue Engineering. J Polym Environ 31, 4738–4751 (2023). https://doi.org/10.1007/s10924-023-02913-y

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