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The Influence of Cooper and Chromium Ions on the Production of Exopolysaccharide and Polyhydroxybutyrate by Rhizobium tropici LBMP-C01

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Abstract

The influence of bivalent copper (Cu2+) and hexavalent chromium (Cr6+) ions on exopolysaccharide (EPS) and polyhydroxybutyrate (PHB) production by the bacterium Rhizobium tropici LBMP-C01 was investigated. The partial structure of both biopolymers produced by rhizobia were characterized, and the quantification of exoR and exoZ gene expression by real-time polymerase chain reaction (Real-Time PCR) was also investigated. Results showed that the supplementation with Cu2+ for 144 h induced an increase in the production of EPS by 48% and PHB by 46.66% compared to the control. Expression studies revealed that the expressed exoR and exoZ genes were affected by metal ion treatment. It can be stated that this particular rhizobial strain is a future potential candidates to produce super adsorbent quite useful for soil bioremediation.

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Acknowledgements

We thank the financial support of CAPES (Coordination of Improvement of Higher Level Personnel), the graduate program in Agricultural Microbiology (Universidade Estadual Paulista, UNESP-FCAV) and the Electronic Microscopy Laboratory (University of São Paulo, USP–Ribeirão Black-SP). The preparation of primer pairs for evaluation of gene expression was performed by Dr. Silvana Pompea of Val de Moraes. In the analyzes of FTIR we counted with the help of Dr Luis Alberto Conalgo, of Embrapa Instrumentação Agropecuária, Brazilian Company of Agricultural Research, São Carlos, Brazil. Also we thank for text review by Dr Manoel Victor Franco Lemos, UNESP Campus Jaboticabal, Brazil.

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Authors and Affiliations

  1. Faculty of Agrarian and Veterinary Sciences, Department of Technology, Laboratory of Biochemistry and Plant Microorganisms, UNESP - Univ Estadual Paulista, Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, 14884-900, Brazil

    Tatiane Fernanda Leonel, Cristiane Moretto, Tereza Cristina Luque Castellane & Eliana Gertrudes de Macedo Lemos

  2. Faculty of Agrarian and Veterinary Sciences, Department of Biology Applied to Agriculture, Laboratory of Genetics of Bacteria and Applied Biotechnology, UNESP - Univ Estadual Paulista, Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, 14884-900, Brazil

    Tereza Cristina Luque Castellane

  3. Faculty of Pharmaceutical Sciences, Laboratory of Clinical Immunology and Molecular Biology, UNESP - Univ Estadual Paulista, Rodovia: Araraquara-Jaú Km 1, Araraquara, Brazil

    Paulo Inácio da Costa

  4. Graduate Program on Agricultural Microbiology, Faculty of Agrarian and Veterinary Sciences, UNESP - Univ Estadual Paulista, Jaboticabal, Brazil

    Tatiane Fernanda Leonel

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  1. Tatiane Fernanda Leonel
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Leonel, T.F., Moretto, C., Castellane, T.C.L. et al. The Influence of Cooper and Chromium Ions on the Production of Exopolysaccharide and Polyhydroxybutyrate by Rhizobium tropici LBMP-C01. J Polym Environ 27, 445–455 (2019). https://doi.org/10.1007/s10924-018-1359-4

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