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Archaeal Poly (3-hydroxybutyrate) Polymer Production from Glycerol: Optimization by Taguchi Methodology

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Abstract

In this study the possibility of poly (3-hydroxybutyrate) production from glycerol was investigated and optimized by Halorcula sp. IRU1, a novel archaea isolated from Urmia lake, Iran in batch experiments. Using Taguchi methodology, three important independent parameters (glycerol, yeast extract and KH2PO4) were evaluated for their individual and interactive effects on poly (3-hydroxybutyrate) production. It was shown that the glycerol concentration was the most significant factor affecting the yield of poly (3-hydroxybutyrate). The optimum factor levels were a glycerol concentration of 8% (v/v), yeast extract 0.8% (w/v) and KH2PO4 0.002% (w/v). The predicted value obtained for poly (3-hydroxybutyrate) production under these conditions was about 81.87%. We can conclude that Haloarcula sp. IRU1 has a high potential for synthesis of poly (3-hydroxybutyrate) from glycerol.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran

    Mojtaba Taran & Elham Azizi

  2. Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran

    Shahein Taran

  3. Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Nadia Asadi

Authors
  1. Mojtaba Taran
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  2. Elham Azizi
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  3. Shahein Taran
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  4. Nadia Asadi
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Correspondence to Mojtaba Taran.

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Taran, M., Azizi, E., Taran, S. et al. Archaeal Poly (3-hydroxybutyrate) Polymer Production from Glycerol: Optimization by Taguchi Methodology. J Polym Environ 19, 750–754 (2011). https://doi.org/10.1007/s10924-011-0327-z

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  • DOI: https://doi.org/10.1007/s10924-011-0327-z

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