Abstract
Acetylcholinesterase (AChE) plays an important role in the treatment of human diseases, environmental security and global food supply. In this study, the simple fluorescent indicators and MnO2 nanosheets were developed and integrated to establish a ratiometric fluorescence sensing system for the detection of AChE activity. Two fluorescence signals could be recorded independently at the same excitation wavelength, which extended the detection range and enhanced the visibility of results. Fluorescence of F-PDA was quenched by MnO2 nanosheets on account of inner filtering effect. Meanwhile, the nonfluorescent OPD was catalytically oxidized to 2,3-diaminophenazine by MnO2 nanosheets. The acetylcholine (ATCh) was catalytically hydrolyzed by AChE to enzymatic thiocholine, which decomposed MnO2 to Mn2+, recovered the fluorescence of F-PDA and reduced the emission of ox-OPD. Utilizing the fluorescence intensity ratio F468/F558 as the signal readout, the ratiometric fluorescence method was established to detect AChE activity. Under the excitation wavelength of 410 nm, the ratio F460/F558 against the AChE concentration demonstrated two linear relationships in the range 0.05 -1.0 and 1.0–50 U·L− 1 with a limit of detection (LOD) of 0.073 U·L− 1. The method was applied to the detection of AChE activity and the analysis of the inhibitor Huperzine-A. Due to the advantages of high sensitivity and favorable selectivity, the method possesses an application prospect in the activity deteceion of AChE and the screening of inhibitors.
Graphical Abstract
Similar content being viewed by others
Data Availability
No datasets were generated or analysed during the current study.
References
Yan X, Song Y, Wu X, Zhu C, Su X, Du D, Lin Y (2017) Nanoscale 9:2317–2323. https://doi.org/10.1039/C6NR08473G
Liu D-M, Xu B, Dong C (2021) TrAC Trends Anal Chem 142:116320. https://doi.org/10.1016/j.trac.2021.116320
Zhang X-P, Xu W, Wang J-H, Shu Y (2022) Analyst 147:4008–4013. https://doi.org/10.1039/D2AN01180H
Pourshojaei Y, Abiri A, Eskandari K, Haghighijoo Z, Edraki N, Asadipour A (2019) Sci Rep 9:19855. https://doi.org/10.1038/s41598-019-56463-2
Liu J, Ma R, Ha W, Zhang H-X, Shi Y-P (2023) Talanta 253. https://doi.org/10.1016/j.talanta.2022.124025
Ye M, Lin B, Yu Y, Li H, Wang Y, Zhang L, Cao Y, Guo M (2020) Microchim Acta 187:511. https://doi.org/10.1007/s00604-020-04522-1
Wang Y, Zhao Q, Xue Y, Wu D, Zhang B, Sun J, Yang X (2023) Sens Actuators B 396:134565. https://doi.org/10.1016/j.snb.2023.134565
Oe M, Miki K, Masuda A, Nogita K, Ohe K (2022) Chem Commun 58:1510–1513. https://doi.org/10.1039/d1cc05132f
Ouyang H, Lu Q, Wang W, Song Y, Tu X, Zhu C, Smith JN, Du D, Fu Z, Lin Y (2018) Anal Chem 90:5147–5152. https://doi.org/10.1021/acs.analchem.7b05247
Chen Q, Yang L-P, Li D-H, Zhai J, Jiang W, Xie X (2021) Sens Actuators B 326:128836. https://doi.org/10.1016/j.snb.2020.128836
Xing L, Ma P, Chen F (2024) Spectrochim Acta Part A 310:123954. https://doi.org/10.1016/j.saa.2024.123954
Yang M, Zhou H, Zhang Y, Hu Z, Niu N, Yu C (2018) Mikrochim Acta 185:132. https://doi.org/10.1007/s00604-018-2678-9
Han Y, Ye Z, Wang F, Chen T, Wei L, Chen L, Xiao L (2019) Nanoscale 11:14793–14801. https://doi.org/10.1039/C9NR01817D
Xiao SJ, Chu ZJ, Zhao XJ, Zhang ZB, Liu YH (2017) Microchim Acta 184:4853–4860. https://doi.org/10.1007/s00604-017-2519-2
Halawa MI, Wu F, Zafar MN, Mostafa IM, Abdussalam A, Han S, Xu G (2020) J Mater Chem B 8:3542–3549. https://doi.org/10.1039/C9TB02158B
Fan D, Shang C, Gu W, Wang E, Dong S (2017) ACS Appl Mater Interfaces 9:25870–25877. https://doi.org/10.1021/acsami.7b07369
Zhang Z, Feng J, Huang P, Li S, Wu F-Y (2019) Sens Actuators B 298. https://doi.org/10.1016/j.snb.2019.126891
Niu C, Liu Q, Shang Z, Zhao L, Ouyang J (2015) Nanoscale 7:8457–8465. https://doi.org/10.1039/c5nr00554j
Huang Z-M, Cai Q-Y, Ding D-C, Ge J, Hu Y-L, Yang J, Zhang L, Li Z-H (2017) Sens Actuators B 242:355–361. https://doi.org/10.1016/j.snb.2016.11.066
Xiao T, Sun J, Zhao J, Wang S, Liu G, Yang X (2018) ACS Appl Mater Interfaces 10:6560–6569. https://doi.org/10.1021/acsami.7b18816
Qu F, Yan H, Li K, You J, Han W (2020) J Mater Sci 55:10022–10034. https://doi.org/10.1007/s10853-020-04754-9
Zhou Q, Zhou T, Tu Y, Yan J (2023) Chem Pap 77:3671–3678. https://doi.org/10.1007/s11696-023-02729-z
Luo L, Fei J, Duan X, Qi Y, Li M, Li H, Huang J (2019) Tribol Int 134:145–153. https://doi.org/10.1016/j.triboint.2019.01.044
Liu Y, Ai K, Lu L (2014) Chem Rev 114:5057–5115. https://doi.org/10.1021/cr400407a
Zhang Y, Wu Y, Liu L, Wang W, Zhang W, Song D, Wang X, Su R (2021) Sens Actuators B 346:130531. https://doi.org/10.1016/j.snb.2021.130531
Zhang J, Yang H, Pan S, Liu H, Hu X (2021) Spectrochim Acta Part A 244. https://doi.org/10.1016/j.saa.2020.118831
Panraksa Y, Siangproh W, Khampieng T, Chailapakul O, Apilux A (2018) Talanta 178:1017–1023. https://doi.org/10.1016/j.talanta.2017.08.096
Han T, Wang G (2019) J Mater Chem B 7:2613–2618. https://doi.org/10.1039/c8tb02616e
Lv J, He B, Wang N, Li M, Lin Y (2018) RSC Adv 8:32893–32898. https://doi.org/10.1039/c8ra06165c
Ni P, Sun Y, Jiang S, Lu W, Wang Y, Li Z, Li Z (2017) Sens Actuators B 240:651–656. https://doi.org/10.1016/j.snb.2016.08.096
Chang J, Li H, Hou T, Li F (2016) Biosens Bioelectron 86:971–977. https://doi.org/10.1016/j.bios.2016.07.022
Liu R, Wu Z, Yang Y, Liao S, Yu R (2018) Mater Res Express 5:065027. https://doi.org/10.1088/2053-1591/aac867
Ye M, Lin B, Yu Y, Li H, Wang Y, Zhang L, Cao Y, Guo M (2020) Mikrochim Acta 187:511. https://doi.org/10.1007/s00604-020-04522-1
Wang M, Liu L, Xie X, Zhou X, Lin Z, Su X (2020) Sens Actuators B 313:128023. https://doi.org/10.1016/j.snb.2020.128023
Wang Y, Xue Y, Zhao Q, Wang S, Sun J, Yang X (2022) Anal Chem 94:16345–16352. https://doi.org/10.1021/acs.analchem.2c03290
Funding
The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.
Author information
Authors and Affiliations
Contributions
FZ (First Author): Conceptualization, Methodology, Software, Investigation, Formal Analysis, Writing - Original Draft, Writing - Review & Editing; HG (Corresponding Author): Conceptualization, Funding Acquisition, Resources, Supervision, Writing - Review & Editing; WY: Data Curation, Writing - Original Draft; LG: Resources, Supervision; JL: Visualization, Investigation; HC: Software, Validation.
Corresponding author
Ethics declarations
Competing Interests
The authors declare no competing interests.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic Supplementary Material
Below is the link to the electronic supplementary material.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Zhao, F., Guo, H., Yang, W. et al. Determination of Acetylcholinesterase Activity Based on Ratiometric Fluorescence Signal Sensing. J Fluoresc (2024). https://doi.org/10.1007/s10895-024-03703-y
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s10895-024-03703-y