Abstract
A method utilizing nitrogen-doped and sulfur-doped carbon quantum dots (N, S-CQDs) as fluorescent probes for the rapid detection of Fe3+, L-ascorbic acid (AA), and alkaline phosphatase (ALP) was presented. The fluorescence intensity of N, S-CQDs nanoprobes can be rapidly and efficiently quenched by Fe3+ and based on the fluorescence “turn off-on” characteristic of N, S-CQDs nanoprobes, the fluorescence signals of the N, S-CQDs/Fe3+can be recovered after the addition of AA. By coupling a fluorescent nanoprobe to an enzyme and L-ascorbic acid-2-phosphate (AA2P), a green, simple, rapid and effective fluorescent analytical method for the determination of ALP was developed. The prepared N, S-CQDs showed high sensitivity and selectivity to Fe3+, AA and ALP with the detection limit of 0.42 μM, 12.7 nM and 0.017 U·L−1 and their optimal concentration ranges were10—600 µM, 10—200 μM, 0.18—54 U·L−1, respectively. The fluorescence quantum yield of N, S-CQDs (0.2 mg·mL−1) at 393 nm excitation wavelength was 4.41%. Additionally, the fluorescent nanoprobes have been employed to successfully measure ALP in serum samples. It is expected that the established method may offer a new approach for biomolecular detection in clinical diagnosis and pharmaceutical analysis.
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References
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Acknowledgements
Associate professor Yang Wang, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road Shenhe District, 110016, Shenyang, Liaoning Province, P. R. China; Shandong Lukang Pharmaceutical Co., Ltd., Jining, Shandong Province, P. R. China.
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This work was supported by the Middle-aged Backbone Personnel Training Program of Shenyang Pharmaceutical University (ZQN2016011) and Science and Technology Department of Liaoning Province(2019-ZD-0450).
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RNW: Conceptualization, Methodology, Data curation, Writing-original draft. YW and NZ: Formal analysis, Investigation, Software, Data curation. HQZ and XCY: Writing-review & Editing, Supervision. Longshan Zhao: Writing-review & Editing, Project administration.All authors reviewed the manuscript.
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Wang, R., Wang, Y., Zhao, N. et al. Nitrogen and Sulfur Co-doped Carbon Quantum Dots for Detecting Fe3+, Ascorbic Acid and Alkaline Phosphatase Activities. J Fluoresc (2023). https://doi.org/10.1007/s10895-023-03539-y
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DOI: https://doi.org/10.1007/s10895-023-03539-y